A phosphorylation tag for uranyl mediated protein purification and photo assisted tag removal.

Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics of e...

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Bibliographic Details
Main Authors: Qiang Zhang, Thomas J D Jørgensen, Peter E Nielsen, Niels Erik Møllegaard
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0091138&type=printable