Protocol for in vivo and ex vivo assessments of glucose-stimulated insulin secretion in mouse islet β cells

Summary: Pancreatic islet β cells secrete insulin in a biphasic manner when sensing high blood glucose level. This protocol describes the evaluation of different phases of insulin secretion, as well as basal, glucose-stimulated and total insulin secretion abilities, thereby enabling precise assessme...

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Detalhes bibliográficos
Principais autores: Yun-Xia Zhu, Yun-Cai Zhou, Yan Zhang, Peng Sun, Xiao-Ai Chang, Xiao Han
Formato: Artigo
Idioma:English
Publicado em: Elsevier 2021-09-01
coleção:STAR Protocols
Assuntos:
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2666166721004354