Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (<i>LcyE</i>) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice

Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (<i>LcyE</i>) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice

Lycopene epsilon-cyclase (<i>LcyE</i>) is a key enzyme in the carotenoid biosynthetic pathway of higher plants. Using the CRSPR/Cas9 and the geminiviral replicon, we optimized a method for targeted mutagenesis and golden SNP replacement of the <i>LcyE</i> gene in rice. We hav...

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Bibliographic Details
Main Authors: Jong Hee Kim, Jihyeon Yu, Hee Kyoung Kim, Jin Young Kim, Me-Sun Kim, Yong-Gu Cho, Sangsu Bae, Kwon Kyoo Kang, Yu Jin Jung
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:International Journal of Molecular Sciences
Subjects:
gene replacement
homology-directed repair (HDR)
CRISPR-Cas9
geminiviral replicons
genome editing
<i>LcyE</i>
Online Access:https://www.mdpi.com/1422-0067/23/18/10383

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https://www.mdpi.com/1422-0067/23/18/10383

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