Long PCR Facilitates Concise Cloning and Sequencing with a Minimal Tiling Set of Templates
We demonstrate a rapid cloning and sequencing strategy for kilobase-size DNA segments using DNase I and long PCR. In a single-tube protocol, deletions were formed in a plasmid insert by two enzymatic cuts, one at a fixed site and one at random. The doubly cut molecules were recircularized to generat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97236st03 |