Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production
To differentiate foot-and-mouth disease (FMD)-infected animals from vaccinated livestock, non-structural proteins (NSPs) must be removed during the FMD vaccine manufacturing process. Currently, NSPs cannot be selectively removed from FMD virus (FMDV) culture supernatant. Therefore, polyethylene glyc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/3/483 |
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| author | Sun Young Park Jung-Min Lee Ah-Young Kim Sang Hyun Park Sim-In Lee Hyejin Kim Jae-Seok Kim Jong-Hyeon Park Young-Joon Ko Choi-Kyu Park |
| author_facet | Sun Young Park Jung-Min Lee Ah-Young Kim Sang Hyun Park Sim-In Lee Hyejin Kim Jae-Seok Kim Jong-Hyeon Park Young-Joon Ko Choi-Kyu Park |
| author_sort | Sun Young Park |
| collection | DOAJ |
| description | To differentiate foot-and-mouth disease (FMD)-infected animals from vaccinated livestock, non-structural proteins (NSPs) must be removed during the FMD vaccine manufacturing process. Currently, NSPs cannot be selectively removed from FMD virus (FMDV) culture supernatant. Therefore, polyethylene glycol (PEG) is utilized to partially separate FMDV from NSPs. However, some NSPs remain in the FMD vaccine, which after repeated immunization, may elicit NSP antibodies in some livestock. To address this drawback, chloroform at a concentration of more than 2% (<i>v/v</i>) was found to remove NSP efficiently without damaging the FMDV particles. Contrary to the PEG-treated vaccine that showed positive NSP antibody responses after the third immunization in goats, the chloroform-treated vaccine did not induce NSP antibodies. In addition to this enhanced vaccine purity, this new method using chloroform could maximize antigen recovery and the vaccine production time could be shortened by two days due to omission of the PEG processing phase. To our knowledge, this is the first report to remove NSPs from FMDV culture supernatant by chemical addition. This novel method could revolutionize the conventional processes of FMD vaccine production. |
| first_indexed | 2024-03-10T16:45:45Z |
| format | Article |
| id | doaj.art-55223f492f604169844f78794108a61c |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T16:45:45Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-55223f492f604169844f78794108a61c2023-11-20T11:36:08ZengMDPI AGVaccines2076-393X2020-08-018348310.3390/vaccines8030483Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine ProductionSun Young Park0Jung-Min Lee1Ah-Young Kim2Sang Hyun Park3Sim-In Lee4Hyejin Kim5Jae-Seok Kim6Jong-Hyeon Park7Young-Joon Ko8Choi-Kyu Park9Animal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaAnimal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaAnimal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaAnimal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaAnimal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaAnimal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaAnimal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaAnimal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaAnimal and Plant Quarantine Agency, Gimcheon-si 39660, KoreaCollege of Veterinary Medicine, Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, KoreaTo differentiate foot-and-mouth disease (FMD)-infected animals from vaccinated livestock, non-structural proteins (NSPs) must be removed during the FMD vaccine manufacturing process. Currently, NSPs cannot be selectively removed from FMD virus (FMDV) culture supernatant. Therefore, polyethylene glycol (PEG) is utilized to partially separate FMDV from NSPs. However, some NSPs remain in the FMD vaccine, which after repeated immunization, may elicit NSP antibodies in some livestock. To address this drawback, chloroform at a concentration of more than 2% (<i>v/v</i>) was found to remove NSP efficiently without damaging the FMDV particles. Contrary to the PEG-treated vaccine that showed positive NSP antibody responses after the third immunization in goats, the chloroform-treated vaccine did not induce NSP antibodies. In addition to this enhanced vaccine purity, this new method using chloroform could maximize antigen recovery and the vaccine production time could be shortened by two days due to omission of the PEG processing phase. To our knowledge, this is the first report to remove NSPs from FMDV culture supernatant by chemical addition. This novel method could revolutionize the conventional processes of FMD vaccine production.https://www.mdpi.com/2076-393X/8/3/483foot-and-mouth disease virusinactivated vaccinenon-structural proteinpuritychloroform |
| spellingShingle | Sun Young Park Jung-Min Lee Ah-Young Kim Sang Hyun Park Sim-In Lee Hyejin Kim Jae-Seok Kim Jong-Hyeon Park Young-Joon Ko Choi-Kyu Park Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production Vaccines foot-and-mouth disease virus inactivated vaccine non-structural protein purity chloroform |
| title | Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production |
| title_full | Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production |
| title_fullStr | Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production |
| title_full_unstemmed | Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production |
| title_short | Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production |
| title_sort | efficient removal of non structural protein using chloroform for foot and mouth disease vaccine production |
| topic | foot-and-mouth disease virus inactivated vaccine non-structural protein purity chloroform |
| url | https://www.mdpi.com/2076-393X/8/3/483 |
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