Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells
Summary: Wnt3a-coated beads can induce asymmetric divisions of mouse embryonic stem cells (mESCs), resulting in one self-renewed mESC and one differentiating epiblast stem cell. This provides an opportunity for studying histone inheritance pattern at a single-cell resolution in cell culture. Here, w...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124720309888 |
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| author | Binbin Ma Tung-Jui Trieu Ji Cheng Shuang Zhou Qingsong Tang Jing Xie Ji-Long Liu Keji Zhao Shukry J. Habib Xin Chen |
| author_facet | Binbin Ma Tung-Jui Trieu Ji Cheng Shuang Zhou Qingsong Tang Jing Xie Ji-Long Liu Keji Zhao Shukry J. Habib Xin Chen |
| author_sort | Binbin Ma |
| collection | DOAJ |
| description | Summary: Wnt3a-coated beads can induce asymmetric divisions of mouse embryonic stem cells (mESCs), resulting in one self-renewed mESC and one differentiating epiblast stem cell. This provides an opportunity for studying histone inheritance pattern at a single-cell resolution in cell culture. Here, we report that mESCs with Wnt3a-bead induction display nonoverlapping preexisting (old) versus newly synthesized (new) histone H3 patterns, but mESCs without Wnt3a beads have largely overlapping patterns. Furthermore, H4K20me2/3, an old histone-enriched modification, displays a higher instance of asymmetric distribution on chromatin fibers from Wnt3a-induced mESCs than those from non-induced mESCs. These locally distinct distributions between old and new histones have both cellular specificity in Wnt3a-induced mESCs and molecular specificity for histones H3 and H4. Given that post-translational modifications at H3 and H4 carry the major histone modifications, our findings provide a mammalian cell culture system to study histone inheritance for maintaining stem cell fate and for resetting it during differentiation. |
| first_indexed | 2024-12-14T22:38:53Z |
| format | Article |
| id | doaj.art-555ef5e88e0d41aaa8c8b1f5a9649eff |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-14T22:38:53Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-555ef5e88e0d41aaa8c8b1f5a9649eff2022-12-21T22:45:03ZengElsevierCell Reports2211-12472020-08-01326108003Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem CellsBinbin Ma0Tung-Jui Trieu1Ji Cheng2Shuang Zhou3Qingsong Tang4Jing Xie5Ji-Long Liu6Keji Zhao7Shukry J. Habib8Xin Chen9Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA; Research Center for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200120, China; Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, ChinaCentre for Stem Cells and Regenerative Medicine, King’s College London, London SE1 9RT, UKDepartment of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA; Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USASchool of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaSystems Biology Center, Division of Intramural Research, NHLBI, NIH, Bethesda, MD, USAResearch Center for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200120, ChinaSchool of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaSystems Biology Center, Division of Intramural Research, NHLBI, NIH, Bethesda, MD, USACentre for Stem Cells and Regenerative Medicine, King’s College London, London SE1 9RT, UKDepartment of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA; Corresponding authorSummary: Wnt3a-coated beads can induce asymmetric divisions of mouse embryonic stem cells (mESCs), resulting in one self-renewed mESC and one differentiating epiblast stem cell. This provides an opportunity for studying histone inheritance pattern at a single-cell resolution in cell culture. Here, we report that mESCs with Wnt3a-bead induction display nonoverlapping preexisting (old) versus newly synthesized (new) histone H3 patterns, but mESCs without Wnt3a beads have largely overlapping patterns. Furthermore, H4K20me2/3, an old histone-enriched modification, displays a higher instance of asymmetric distribution on chromatin fibers from Wnt3a-induced mESCs than those from non-induced mESCs. These locally distinct distributions between old and new histones have both cellular specificity in Wnt3a-induced mESCs and molecular specificity for histones H3 and H4. Given that post-translational modifications at H3 and H4 carry the major histone modifications, our findings provide a mammalian cell culture system to study histone inheritance for maintaining stem cell fate and for resetting it during differentiation.http://www.sciencedirect.com/science/article/pii/S2211124720309888histoneasymmetric cell divisionmouse embryonic stem cellsWnt3a beads |
| spellingShingle | Binbin Ma Tung-Jui Trieu Ji Cheng Shuang Zhou Qingsong Tang Jing Xie Ji-Long Liu Keji Zhao Shukry J. Habib Xin Chen Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells Cell Reports histone asymmetric cell division mouse embryonic stem cells Wnt3a beads |
| title | Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells |
| title_full | Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells |
| title_fullStr | Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells |
| title_full_unstemmed | Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells |
| title_short | Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells |
| title_sort | differential histone distribution patterns in induced asymmetrically dividing mouse embryonic stem cells |
| topic | histone asymmetric cell division mouse embryonic stem cells Wnt3a beads |
| url | http://www.sciencedirect.com/science/article/pii/S2211124720309888 |
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