Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery

Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery

Summary: Stop codon suppression using dedicated tRNA/aminoacyl-tRNA synthetase (aaRS) pairs allows for genetically encoded, site-specific incorporation of non-canonical amino acids (ncAAs) as chemical handles for protein labeling and modification. Here, we demonstrate that piggyBac-mediated genomic...

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Bibliographic Details
Main Authors: Birthe Meineke, Johannes Heimgärtner, Rozina Caridha, Matthias F. Block, Kyle J. Kimler, Maria F. Pires, Michael Landreh, Simon J. Elsässer
Format: Article
Language:English
Published: Elsevier 2023-11-01
Series:Cell Reports: Methods
Subjects:
CP: Biotechnology
CP: Molecular biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2667237523002904

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http://www.sciencedirect.com/science/article/pii/S2667237523002904

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