An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation

An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation

Fluorescent reporter mouse lines and Cre/Flp recombinase driver lines play essential roles in investigating various molecular functions <i>in vivo</i>. Now that applications of the CRISPR/Cas9 genome-editing system to mouse fertilized eggs have drastically accelerated these knock-in mous...

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Bibliographic Details
Main Authors: Yukiko U. Inoue, Yuki Morimoto, Mayumi Yamada, Ryosuke Kaneko, Kazumi Shimaoka, Shinji Oki, Mayuko Hotta, Junko Asami, Eriko Koike, Kei Hori, Mikio Hoshino, Itaru Imayoshi, Takayoshi Inoue
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Cells
Subjects:
long ssDNA
phospho-PCR
knock-in
CRISPR/Cas9
Online Access:https://www.mdpi.com/2073-4409/10/5/1076

Internet

https://www.mdpi.com/2073-4409/10/5/1076

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