Polysaccharides from <i>Holothuria leucospilota</i> Relieve Loperamide-Induced Constipation Symptoms in Mice
A vital bioactive component of marine resources is <i>Holothuria leucospilota</i> polysaccharides (HLP). This study examined whether HLP could regulate intestinal flora to treat loperamide-induced constipation. Constipated mice showed signs of prolonged defecation (up by 60.79 min) and a...
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2553 |
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| author | Ziqi Wang Yali Shi Shiyu Zeng Yuanping Zheng Huaijie Wang Haihui Liao Jie Song Xinyue Zhang Jun Cao Chuan Li |
| author_facet | Ziqi Wang Yali Shi Shiyu Zeng Yuanping Zheng Huaijie Wang Haihui Liao Jie Song Xinyue Zhang Jun Cao Chuan Li |
| author_sort | Ziqi Wang |
| collection | DOAJ |
| description | A vital bioactive component of marine resources is <i>Holothuria leucospilota</i> polysaccharides (HLP). This study examined whether HLP could regulate intestinal flora to treat loperamide-induced constipation. Constipated mice showed signs of prolonged defecation (up by 60.79 min) and a reduced number of bowel movements and pellet water content (decreased by 12.375 and 11.77%, respectively). The results showed that HLP treatment reduced these symptoms, reversed the changes in related protein expression levels in the colon, and regulated the levels of active peptides associated with the gastrointestinal tract in constipated mice, which significantly improved water-electrolyte metabolism and enhanced gastrointestinal motility. Meanwhile, it was found that intestinal barrier damage was reduced and the inflammatory response was inhibited through histopathology and immunohistochemistry. As a means to further relieve constipation symptoms, treatment with low, medium, and high HLP concentrations increased the total short-chain fatty acid (SCFA) content in the intestine of constipated mice by 62.60 μg/g, 138.91 μg/g, and 126.51 μg/g, respectively. Moreover, an analysis of the intestinal flora’s gene for 16S rRNA suggested that the intestinal microbiota was improved through HLP treatment, which is relevant to the motivation for the production of SCFAs. In summary, it was demonstrated that HLP reduced loperamide-induced constipation in mice. |
| first_indexed | 2024-03-11T09:40:47Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:40:47Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-55ccac8e300b460abe78c0377e9926a02023-11-16T16:58:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243255310.3390/ijms24032553Polysaccharides from <i>Holothuria leucospilota</i> Relieve Loperamide-Induced Constipation Symptoms in MiceZiqi Wang0Yali Shi1Shiyu Zeng2Yuanping Zheng3Huaijie Wang4Haihui Liao5Jie Song6Xinyue Zhang7Jun Cao8Chuan Li9Key Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaA vital bioactive component of marine resources is <i>Holothuria leucospilota</i> polysaccharides (HLP). This study examined whether HLP could regulate intestinal flora to treat loperamide-induced constipation. Constipated mice showed signs of prolonged defecation (up by 60.79 min) and a reduced number of bowel movements and pellet water content (decreased by 12.375 and 11.77%, respectively). The results showed that HLP treatment reduced these symptoms, reversed the changes in related protein expression levels in the colon, and regulated the levels of active peptides associated with the gastrointestinal tract in constipated mice, which significantly improved water-electrolyte metabolism and enhanced gastrointestinal motility. Meanwhile, it was found that intestinal barrier damage was reduced and the inflammatory response was inhibited through histopathology and immunohistochemistry. As a means to further relieve constipation symptoms, treatment with low, medium, and high HLP concentrations increased the total short-chain fatty acid (SCFA) content in the intestine of constipated mice by 62.60 μg/g, 138.91 μg/g, and 126.51 μg/g, respectively. Moreover, an analysis of the intestinal flora’s gene for 16S rRNA suggested that the intestinal microbiota was improved through HLP treatment, which is relevant to the motivation for the production of SCFAs. In summary, it was demonstrated that HLP reduced loperamide-induced constipation in mice.https://www.mdpi.com/1422-0067/24/3/2553<i>Holothuria leucospilota</i> polysaccharidesconstipationhormoneshort-chain fatty acidgut microbiota |
| spellingShingle | Ziqi Wang Yali Shi Shiyu Zeng Yuanping Zheng Huaijie Wang Haihui Liao Jie Song Xinyue Zhang Jun Cao Chuan Li Polysaccharides from <i>Holothuria leucospilota</i> Relieve Loperamide-Induced Constipation Symptoms in Mice International Journal of Molecular Sciences <i>Holothuria leucospilota</i> polysaccharides constipation hormone short-chain fatty acid gut microbiota |
| title | Polysaccharides from <i>Holothuria leucospilota</i> Relieve Loperamide-Induced Constipation Symptoms in Mice |
| title_full | Polysaccharides from <i>Holothuria leucospilota</i> Relieve Loperamide-Induced Constipation Symptoms in Mice |
| title_fullStr | Polysaccharides from <i>Holothuria leucospilota</i> Relieve Loperamide-Induced Constipation Symptoms in Mice |
| title_full_unstemmed | Polysaccharides from <i>Holothuria leucospilota</i> Relieve Loperamide-Induced Constipation Symptoms in Mice |
| title_short | Polysaccharides from <i>Holothuria leucospilota</i> Relieve Loperamide-Induced Constipation Symptoms in Mice |
| title_sort | polysaccharides from i holothuria leucospilota i relieve loperamide induced constipation symptoms in mice |
| topic | <i>Holothuria leucospilota</i> polysaccharides constipation hormone short-chain fatty acid gut microbiota |
| url | https://www.mdpi.com/1422-0067/24/3/2553 |
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