DIA label-free proteomic analysis of murine bone-marrow-derived macrophages

DIA label-free proteomic analysis of murine bone-marrow-derived macrophages

Summary: Here, we describe an optimized protocol to analyze murine bone-marrow-derived macrophages using label-free data-independent acquisition (DIA) proteomics. We provide a complete step-by-step protocol describing sample preparation utilizing the S-Trap approach for on-column digestion and pepti...

Full description

Bibliographic Details
Main Authors: Christa P. Baker, Iain R. Phair, Alejandro J. Brenes, Abdelmadjid Atrih, Dylan G. Ryan, Roland Bruderer, Albena T. Dinkova-Kostova, Douglas J. Lamont, J. Simon C. Arthur, Andrew J.M. Howden
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Bioinformatics
Immunology
Protein biochemistry
Proteomics
Mass spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006050

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722006050

Similar Items