DIA label-free proteomic analysis of murine bone-marrow-derived macrophages

DIA label-free proteomic analysis of murine bone-marrow-derived macrophages

Summary: Here, we describe an optimized protocol to analyze murine bone-marrow-derived macrophages using label-free data-independent acquisition (DIA) proteomics. We provide a complete step-by-step protocol describing sample preparation utilizing the S-Trap approach for on-column digestion and pepti...

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Bibliographic Details
Main Authors:	Christa P. Baker, Iain R. Phair, Alejandro J. Brenes, Abdelmadjid Atrih, Dylan G. Ryan, Roland Bruderer, Albena T. Dinkova-Kostova, Douglas J. Lamont, J. Simon C. Arthur, Andrew J.M. Howden
Format:	Article
Language:	English
Published:	Elsevier 2022-12-01
Series:	STAR Protocols
Subjects:	Bioinformatics Immunology Protein biochemistry Proteomics Mass spectrometry
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722006050

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