16S rRNA-Based Microbiota Profiling Assists Conventional Culture Analysis of Airway Samples from Pediatric Cystic Fibrosis Patients

16S rRNA-Based Microbiota Profiling Assists Conventional Culture Analysis of Airway Samples from Pediatric Cystic Fibrosis Patients

ABSTRACT 16S-based sequencing provides broader information on the respiratory microbial community than conventional culturing. However, it (often) lacks species- and strain-level information. To overcome this issue, we used 16S rRNA-based sequencing results from 246 nasopharyngeal samples obtained f...

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Bibliographic Details
Main Authors: Maartje Kristensen, Emma M. de Koff, Mei Ling Chu, Simone Groendijk, Gerdien A. Tramper-Stranders, Karin M. de Winter-de Groot, Hettie M. Janssens, Harm A. Tiddens, Mireille van Westreenen, Elisabeth A. M. Sanders, Bert H. G. M. Arets, Cornelis K. van der Ent, Sabine M. P. J. Prevaes, Debby Bogaert
Format: Article
Language:English
Published: American Society for Microbiology 2023-06-01
Series:Microbiology Spectrum
Subjects:
16S rRNA sequencing
bacterial culture
cystic fibrosis
microbiome
Online Access:https://journals.asm.org/doi/10.1128/spectrum.04057-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.04057-22

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