Differentiation of human mesenchymal stem cell spheroids under microgravity conditions

To develop and characterize a novel cell culture method for the generation of undifferentiated and differentiated human mesenchymal stem cell 3D structures, we utilized the RWV system with a gelatin-based scaffold. 3 × 106 cells generated homogeneous spheroids and maximum spheroid loading was accomp...

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Bibliographic Details
Main Authors: Wolfgang H Cerwinka, Starlette M Sharp, Barbara D Boyan, Haiyen E Zhau, Leland W K Chung, Clayton Yates
Format: Article
Language:English
Published: SpringerOpen 2012-01-01
Series:Cell Regeneration
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2045976917300305