ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a
Abstract Background We have reported that the phosphatidylinositol-3 kinase (PI3K)/Akt/RhoA signaling pathway mediates Wnt5a-induced cell migration of osteosarcoma cells. However, the specific receptors responding to Wnt5a ligand remain poorly defined in osteosarcoma metastasis. Methods Wound healin...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12935-017-0482-y |
| _version_ | 1828770402907717632 |
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| author | Bin Dai Ting Yan Ailiang Zhang |
| author_facet | Bin Dai Ting Yan Ailiang Zhang |
| author_sort | Bin Dai |
| collection | DOAJ |
| description | Abstract Background We have reported that the phosphatidylinositol-3 kinase (PI3K)/Akt/RhoA signaling pathway mediates Wnt5a-induced cell migration of osteosarcoma cells. However, the specific receptors responding to Wnt5a ligand remain poorly defined in osteosarcoma metastasis. Methods Wound healing assays were used to measure the migration rate of osteosarcoma cells transfected with shRNA or siRNA specific against ROR2 or indicated constructs. We evaluated the RhoA activation in osteosarcoma MG-63 and U2OS cells with RhoA activation assay. A panel of inhibitors of PI3K and Akt treated osteosarcoma cells and blocked kinase activity. Western blotting assays were employed to measure the expression and activation of Akt. Clonogenic assays were used to measure the cell proliferation of ROR2-knockdown or ROR2-overexpressed osteosarcoma cells. Results Wnt5a-induced osteosarcoma cell migration was largely abolished by shRNA or siRNA specific against ROR2. Overexpression of RhoA-CA (GFP-RhoA-V14) was able to rescue the Wnt5a-induced cell migration blocked by ROR2 knockdown. The Wnt5a-induced activation of RhoA was mostly blocked by ROR2 knockdown, and elevated by ROR2 overexpression, respectively. Furthermore, we found that Wnt5a-induced cell migration was significantly retarded by RhoA-siRNA transfection or pretreatment of HS-173 (PI3Kα inhibitor), MK-2206 (Akt inhibitor), A-674563 (Akt1 inhibitor), or CCT128930 (Akt2 inhibitor). The activation of Akt was upregulated or downregulated by transfected with ROR2-Flag or ROR2-siRNA, respectively. Lastly, Wnt5a/ROR2 signaling does not alter the cell proliferation of MG-63 osteosarcoma cells. Conclusions Taken together, we demonstrate that ROR2 receptor responding to Wnt5a ligand activates PI3K/Akt/RhoA signaling and promotes the migration of osteosarcoma cells. |
| first_indexed | 2024-12-11T14:06:25Z |
| format | Article |
| id | doaj.art-55e8faca8f394ae5a74d4e7692383f9f |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-11T14:06:25Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-55e8faca8f394ae5a74d4e7692383f9f2022-12-22T01:03:38ZengBMCCancer Cell International1475-28672017-11-011711910.1186/s12935-017-0482-yROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5aBin Dai0Ting Yan1Ailiang Zhang2Department of Orthopedics, Binhai County People’s HospitalSafety Assessment and Research Center for Drug, Pesticide and Veterinary Drug of Jiangsu Province, School of Public Health, Nanjing Medical UniversitySpine Surgery, Third Affiliated Hospital of Soochow UniversityAbstract Background We have reported that the phosphatidylinositol-3 kinase (PI3K)/Akt/RhoA signaling pathway mediates Wnt5a-induced cell migration of osteosarcoma cells. However, the specific receptors responding to Wnt5a ligand remain poorly defined in osteosarcoma metastasis. Methods Wound healing assays were used to measure the migration rate of osteosarcoma cells transfected with shRNA or siRNA specific against ROR2 or indicated constructs. We evaluated the RhoA activation in osteosarcoma MG-63 and U2OS cells with RhoA activation assay. A panel of inhibitors of PI3K and Akt treated osteosarcoma cells and blocked kinase activity. Western blotting assays were employed to measure the expression and activation of Akt. Clonogenic assays were used to measure the cell proliferation of ROR2-knockdown or ROR2-overexpressed osteosarcoma cells. Results Wnt5a-induced osteosarcoma cell migration was largely abolished by shRNA or siRNA specific against ROR2. Overexpression of RhoA-CA (GFP-RhoA-V14) was able to rescue the Wnt5a-induced cell migration blocked by ROR2 knockdown. The Wnt5a-induced activation of RhoA was mostly blocked by ROR2 knockdown, and elevated by ROR2 overexpression, respectively. Furthermore, we found that Wnt5a-induced cell migration was significantly retarded by RhoA-siRNA transfection or pretreatment of HS-173 (PI3Kα inhibitor), MK-2206 (Akt inhibitor), A-674563 (Akt1 inhibitor), or CCT128930 (Akt2 inhibitor). The activation of Akt was upregulated or downregulated by transfected with ROR2-Flag or ROR2-siRNA, respectively. Lastly, Wnt5a/ROR2 signaling does not alter the cell proliferation of MG-63 osteosarcoma cells. Conclusions Taken together, we demonstrate that ROR2 receptor responding to Wnt5a ligand activates PI3K/Akt/RhoA signaling and promotes the migration of osteosarcoma cells.http://link.springer.com/article/10.1186/s12935-017-0482-yROR2Wnt5aOsteosarcomaMigration |
| spellingShingle | Bin Dai Ting Yan Ailiang Zhang ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a Cancer Cell International ROR2 Wnt5a Osteosarcoma Migration |
| title | ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a |
| title_full | ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a |
| title_fullStr | ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a |
| title_full_unstemmed | ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a |
| title_short | ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a |
| title_sort | ror2 receptor promotes the migration of osteosarcoma cells in response to wnt5a |
| topic | ROR2 Wnt5a Osteosarcoma Migration |
| url | http://link.springer.com/article/10.1186/s12935-017-0482-y |
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