Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines

Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines

Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified 3T3-L1 cell line overexpressing Cas9 for a straig...

Full description

Bibliographic Details
Main Authors: Tomás Suchý, Isabell Kaczmarek, Tomislav Maricic, Christian Zieschang, Torsten Schöneberg, Doreen Thor, Ines Liebscher
Format: Article
Language:English
Published: Taylor & Francis Group 2021-01-01
Series:Adipocyte
Subjects:
adipocytes
cas9
crispr/cas
adipocyte function
3t3-l1
genetic knock-out
Online Access:http://dx.doi.org/10.1080/21623945.2021.1990480

Internet

http://dx.doi.org/10.1080/21623945.2021.1990480

Similar Items