Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid

Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid

Abstract Background The purpose of this study was to evaluate different pretreatment, extraction, amplification, and library generation methods for metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) and to develop an efficient procedure for the simultaneous detection of DNA a...

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Bibliographic Details
Main Authors: Hanfang Jiang, Zhihao Xing, Xiaorong Liu, Qiang Chai, Zefeng Xin, Chunqing Zhu, Ruihong Lin, Xuwen Deng, Dong Cui, HongDan Gao, Dongli Ma
Format: Article
Language:English
Published: BMC 2022-04-01
Series:BMC Infectious Diseases
Subjects:
Encephalitis
Next-generation sequencing
Metagenomic diagnosis
Online Access:https://doi.org/10.1186/s12879-022-07272-y

Internet

https://doi.org/10.1186/s12879-022-07272-y

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