Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells

Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells

Protein-protein interaction studies provide valuable insights into cellular signaling. Brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Brassinosteroid Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase 1 (BAK1). BRI1 and BAK1 were shown to interact independen...

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Bibliographic Details
Main Authors: Nina Glöckner, Sven zur Oven-Krockhaus, Leander Rohr, Frank Wackenhut, Moritz Burmeister, Friederike Wanke, Eleonore Holzwart, Alfred J. Meixner, Sebastian Wolf, Klaus Harter
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Plants
Subjects:
protein-protein interaction
plasma membrane
nanodomains
spectral Förster resonance energy transfer (FRET)
FRET-fluorescence lifetime imaging microscopy (FRET-FLIM)
Online Access:https://www.mdpi.com/2223-7747/11/19/2630

Internet

https://www.mdpi.com/2223-7747/11/19/2630

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