Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet
IntroductionThe Tibetan Plateau is characterized by low temperature and hypoxia. N-carbamylglutamic acid (NCG) can increase blood oxygen saturation, and have the potential to be used to prevent the high-altitude hypoxia stress state of cows. However, its beneficial effect on the rumen microbiota of...
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| Format: | Article |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1101620/full |
| _version_ | 1797830968653905920 |
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| author | Jinjia Zhu Yicheng Wu Yicheng Wu Aoyu Jiang Aoyu Jiang Bin Li Tao Ran Chuanshe Zhou Chuanshe Zhou Dingfu Xiao Zhiliang Tan Zhiliang Tan |
| author_facet | Jinjia Zhu Yicheng Wu Yicheng Wu Aoyu Jiang Aoyu Jiang Bin Li Tao Ran Chuanshe Zhou Chuanshe Zhou Dingfu Xiao Zhiliang Tan Zhiliang Tan |
| author_sort | Jinjia Zhu |
| collection | DOAJ |
| description | IntroductionThe Tibetan Plateau is characterized by low temperature and hypoxia. N-carbamylglutamic acid (NCG) can increase blood oxygen saturation, and have the potential to be used to prevent the high-altitude hypoxia stress state of cows. However, its beneficial effect on the rumen microbiota of Holstein dairy cows remains unclear.MethodsHence, the experiments 12 multiparous (parity ranged from 2 to 7) Holstein dairy cows (413.0 ± 42 kg) were randomly assigned to 2 treatments with 6 replicates in each treatment: basal diet (CON, control group) and basal diet plus 20 g/d/cow of NCG (NCG, experiment group), respectively. To study the effects of dietary NCG supplementation on rumen microbiota of Holstein dairy cows in Tibet. The experiment lasted for 45 days, with 15 days of pre-feeding and 30 days of formal trail period.ResultsThe results showed that ruminal NH3-N concentration in NCG group was lower (p < 0.05) than that in the CON group, while molar proportion of acetic acid and total volatile fatty acid (VFA) concentration were increased (p < 0.05) with the addition of NCG. Microbial diversity increased (p < 0.05) in NCG group, with Bacteroidetes, Firmicutes, and Patescibacteria as the most abundant phyla. The KEGG pathway analysis showed that the potential function of ruminal bacteria was mainly enriched in metabolism (carbohydrates, amino acids, lipids, energy, and nucleotides) and genetic information processing (replication, repair, and translation).ConclusionIn conclusion, NCG can improve rumen nitrogen utilization, total VFA and acetic acid production, and increase rumen microbial diversity, all of which could make the introduced Holstein dairy cows to better adapt to the harsh environment in Tibet and improve their production performance. |
| first_indexed | 2024-04-09T13:45:16Z |
| format | Article |
| id | doaj.art-564390a791d748ab8ab091d1f7a3812c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T13:45:16Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-564390a791d748ab8ab091d1f7a3812c2023-05-09T05:06:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11016201101620Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in TibetJinjia Zhu0Yicheng Wu1Yicheng Wu2Aoyu Jiang3Aoyu Jiang4Bin Li5Tao Ran6Chuanshe Zhou7Chuanshe Zhou8Dingfu Xiao9Zhiliang Tan10Zhiliang Tan11CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Advanced Agricultural, University of the Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Advanced Agricultural, University of the Chinese Academy of Sciences, Beijing, ChinaInstitute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Lhasa, Tibet, ChinaCollege of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Advanced Agricultural, University of the Chinese Academy of Sciences, Beijing, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Advanced Agricultural, University of the Chinese Academy of Sciences, Beijing, ChinaIntroductionThe Tibetan Plateau is characterized by low temperature and hypoxia. N-carbamylglutamic acid (NCG) can increase blood oxygen saturation, and have the potential to be used to prevent the high-altitude hypoxia stress state of cows. However, its beneficial effect on the rumen microbiota of Holstein dairy cows remains unclear.MethodsHence, the experiments 12 multiparous (parity ranged from 2 to 7) Holstein dairy cows (413.0 ± 42 kg) were randomly assigned to 2 treatments with 6 replicates in each treatment: basal diet (CON, control group) and basal diet plus 20 g/d/cow of NCG (NCG, experiment group), respectively. To study the effects of dietary NCG supplementation on rumen microbiota of Holstein dairy cows in Tibet. The experiment lasted for 45 days, with 15 days of pre-feeding and 30 days of formal trail period.ResultsThe results showed that ruminal NH3-N concentration in NCG group was lower (p < 0.05) than that in the CON group, while molar proportion of acetic acid and total volatile fatty acid (VFA) concentration were increased (p < 0.05) with the addition of NCG. Microbial diversity increased (p < 0.05) in NCG group, with Bacteroidetes, Firmicutes, and Patescibacteria as the most abundant phyla. The KEGG pathway analysis showed that the potential function of ruminal bacteria was mainly enriched in metabolism (carbohydrates, amino acids, lipids, energy, and nucleotides) and genetic information processing (replication, repair, and translation).ConclusionIn conclusion, NCG can improve rumen nitrogen utilization, total VFA and acetic acid production, and increase rumen microbial diversity, all of which could make the introduced Holstein dairy cows to better adapt to the harsh environment in Tibet and improve their production performance.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1101620/fullN-carbamylglutamaterumen bacteriarumen fermentationTibetHolstein dairy cow |
| spellingShingle | Jinjia Zhu Yicheng Wu Yicheng Wu Aoyu Jiang Aoyu Jiang Bin Li Tao Ran Chuanshe Zhou Chuanshe Zhou Dingfu Xiao Zhiliang Tan Zhiliang Tan Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet Frontiers in Microbiology N-carbamylglutamate rumen bacteria rumen fermentation Tibet Holstein dairy cow |
| title | Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet |
| title_full | Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet |
| title_fullStr | Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet |
| title_full_unstemmed | Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet |
| title_short | Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet |
| title_sort | effects of dietary n carbamylglutamate on rumen fermentation parameters and bacterial community diversity of holstein dairy cows in tibet |
| topic | N-carbamylglutamate rumen bacteria rumen fermentation Tibet Holstein dairy cow |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1101620/full |
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