HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains
IntroductionShort-chain fatty acids (SCFAs) are the main by-products of microbial fermentations occurring in the human intestine and are directly involved in the host’s physiological balance. As impaired gut concentrations of acetic, propionic, and butyric acids are often associated with systemic di...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1124144/full |
| _version_ | 1827999699009798144 |
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| author | Marco Calvigioni Andrea Bertolini Simone Codini Diletta Mazzantini Adelaide Panattoni Mariacristina Massimino Francesco Celandroni Riccardo Zucchi Alessandro Saba Emilia Ghelardi Emilia Ghelardi |
| author_facet | Marco Calvigioni Andrea Bertolini Simone Codini Diletta Mazzantini Adelaide Panattoni Mariacristina Massimino Francesco Celandroni Riccardo Zucchi Alessandro Saba Emilia Ghelardi Emilia Ghelardi |
| author_sort | Marco Calvigioni |
| collection | DOAJ |
| description | IntroductionShort-chain fatty acids (SCFAs) are the main by-products of microbial fermentations occurring in the human intestine and are directly involved in the host’s physiological balance. As impaired gut concentrations of acetic, propionic, and butyric acids are often associated with systemic disorders, the administration of SCFA-producing microorganisms has been suggested as attractive approach to solve symptoms related to SCFA deficiency.MethodsIn this research, nine probiotic strains (Bacillus clausii NR, OC, SIN, and T, Bacillus coagulans ATCC 7050, Bifidobacterium breve DSM 16604, Limosilactobacillus reuteri DSM 17938, Lacticaseibacillus rhamnosus ATCC 53103, and Saccharomyces boulardii CNCM I-745) commonly included in commercial formulations were tested for their ability to secrete SCFAs by using an improved protocol in high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS-MS).ResultsThe developed method was highly sensitive and specific, showing excellent limits of detection and quantification of secreted SCFAs. All tested microorganisms were shown to secrete acetic acid, with only B. clausii and S. boulardii additionally able to produce propionic and butyric acids. Quantitative differences in the secretion of SCFAs were also evidenced.DiscussionThe experimental approach described in this study may contribute to the characterization of probiotics as SCFA-producing organisms, a crucial stage toward their application to improve SCFA deficiency. |
| first_indexed | 2024-04-10T06:03:48Z |
| format | Article |
| id | doaj.art-564555345a0f443e95bc1e77e13fd8a6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T06:03:48Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-564555345a0f443e95bc1e77e13fd8a62023-03-03T05:26:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11241441124144HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strainsMarco Calvigioni0Andrea Bertolini1Simone Codini2Diletta Mazzantini3Adelaide Panattoni4Mariacristina Massimino5Francesco Celandroni6Riccardo Zucchi7Alessandro Saba8Emilia Ghelardi9Emilia Ghelardi10Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, ItalyDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, ItalyDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, ItalyDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, ItalyDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, ItalyResearch Center Nutraceuticals and Food for Health–Nutrafood, University of Pisa, Pisa, ItalyIntroductionShort-chain fatty acids (SCFAs) are the main by-products of microbial fermentations occurring in the human intestine and are directly involved in the host’s physiological balance. As impaired gut concentrations of acetic, propionic, and butyric acids are often associated with systemic disorders, the administration of SCFA-producing microorganisms has been suggested as attractive approach to solve symptoms related to SCFA deficiency.MethodsIn this research, nine probiotic strains (Bacillus clausii NR, OC, SIN, and T, Bacillus coagulans ATCC 7050, Bifidobacterium breve DSM 16604, Limosilactobacillus reuteri DSM 17938, Lacticaseibacillus rhamnosus ATCC 53103, and Saccharomyces boulardii CNCM I-745) commonly included in commercial formulations were tested for their ability to secrete SCFAs by using an improved protocol in high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS-MS).ResultsThe developed method was highly sensitive and specific, showing excellent limits of detection and quantification of secreted SCFAs. All tested microorganisms were shown to secrete acetic acid, with only B. clausii and S. boulardii additionally able to produce propionic and butyric acids. Quantitative differences in the secretion of SCFAs were also evidenced.DiscussionThe experimental approach described in this study may contribute to the characterization of probiotics as SCFA-producing organisms, a crucial stage toward their application to improve SCFA deficiency.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1124144/fullprobioticsshort-chain fatty acidsacetic acidpropionic acidbutyric acidsecretion |
| spellingShingle | Marco Calvigioni Andrea Bertolini Simone Codini Diletta Mazzantini Adelaide Panattoni Mariacristina Massimino Francesco Celandroni Riccardo Zucchi Alessandro Saba Emilia Ghelardi Emilia Ghelardi HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains Frontiers in Microbiology probiotics short-chain fatty acids acetic acid propionic acid butyric acid secretion |
| title | HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains |
| title_full | HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains |
| title_fullStr | HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains |
| title_full_unstemmed | HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains |
| title_short | HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains |
| title_sort | hplc ms ms quantification of short chain fatty acids actively secreted by probiotic strains |
| topic | probiotics short-chain fatty acids acetic acid propionic acid butyric acid secretion |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1124144/full |
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