High-Throughput Large-Scale Targeted Proteomics Assays for Quantifying Pathway Proteins in Pseudomonas putida KT2440

Targeted proteomics is a mass spectrometry-based protein quantification technique with high sensitivity, accuracy, and reproducibility. As a key component in the multi-omics toolbox of systems biology, targeted liquid chromatography-selected reaction monitoring (LC-SRM) measurements are critical for...

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Bibliographic Details
Main Authors: Yuqian Gao, Thomas L. Fillmore, Nathalie Munoz, Gayle J. Bentley, Christopher W. Johnson, Joonhoon Kim, Jamie A. Meadows, Jeremy D. Zucker, Meagan C. Burnet, Anna K. Lipton, Aivett Bilbao, Daniel J. Orton, Young-Mo Kim, Ronald J. Moore, Errol W. Robinson, Scott E. Baker, Bobbie-Jo M. Webb-Robertson, Adam M. Guss, John M. Gladden, Gregg T. Beckham, Jon K. Magnuson, Kristin E. Burnum-Johnson
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.603488/full