Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

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Detalhes bibliográficos
Main Authors:	Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Formato:	Artigo
Idioma:	English
Publicado em:	Wiley 2021-07-01
Colecção:	Ecology and Evolution
Assuntos:	amplicon sequencing biomonitoring bulk sample Malaise trap PCR bias
Acesso em linha:	https://doi.org/10.1002/ece3.7753

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