Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

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Chi tiết về thư mục
Những tác giả chính: Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Định dạng: Bài viết
Ngôn ngữ:English
Được phát hành: Wiley 2021-07-01
Loạt:Ecology and Evolution
Những chủ đề:
amplicon sequencing
biomonitoring
bulk sample
Malaise trap
PCR bias
Truy cập trực tuyến:https://doi.org/10.1002/ece3.7753

Internet

https://doi.org/10.1002/ece3.7753

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