Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

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Main Authors: Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
格式: 文件
语言:English
出版: Wiley 2021-07-01
丛编:Ecology and Evolution
主题:
在线阅读:https://doi.org/10.1002/ece3.7753