| Summary: | <i>Alternaria</i> spp. and its toxins are the main contaminants in processing tomato. Based on our earlier research, the current study looked into the anti-fungal capacity of crude lipopeptides from <i>B. amyloliquefaciens</i> XJ-BV2007 against <i>A. alternata</i>. We found that the crude lipopeptides significantly inhibited <i>A. alternata</i> growth and reduced tomato black spot disease incidence. SEM analysis found that the crude lipopeptides could change the morphology of mycelium and spores of <i>A. alternata</i>. Four main <i>Alternaria</i> toxins were detected using UPLC-MS/MS, and the findings demonstrated that the crude lipopeptides could lessen the accumulation of <i>Alternaria</i> toxins in vivo and in vitro. Meanwhile, under the stress of crude lipopeptides, the expression of critical biosynthetic genes responsible for TeA, AOH, and AME was substantially down-regulated. The inhibitory mechanism of the crude lipopeptides was demonstrated to be the disruption of the mycelial structure of <i>A. alternata</i>, as well as the integrity and permeability of the membrane of <i>A. alternata</i> sporocytes. Taken together, crude lipopeptides extracted from <i>B. amyloliquefaciens</i> XJ-BV2007 are an effective biological agent for controlling tomato black spot disease and <i>Alternaria</i> toxins contamination.
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