Crude Lipopeptides Produced by <i>Bacillus amyloliquefaciens</i> Could Control the Growth of <i>Alternaria alternata</i> and Production of <i>Alternaria</i> Toxins in Processing Tomato
<i>Alternaria</i> spp. and its toxins are the main contaminants in processing tomato. Based on our earlier research, the current study looked into the anti-fungal capacity of crude lipopeptides from <i>B. amyloliquefaciens</i> XJ-BV2007 against <i>A. alternata</i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/16/2/65 |
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| author | Yuanyuan Zhang Yingying Fan Yingying Dai Qinlan Jia Ying Guo Peicheng Wang Tingting Shen Yan Wang Fengjuan Liu Wanhui Guo Aibo Wu Ziwei Jiao Cheng Wang |
| author_facet | Yuanyuan Zhang Yingying Fan Yingying Dai Qinlan Jia Ying Guo Peicheng Wang Tingting Shen Yan Wang Fengjuan Liu Wanhui Guo Aibo Wu Ziwei Jiao Cheng Wang |
| author_sort | Yuanyuan Zhang |
| collection | DOAJ |
| description | <i>Alternaria</i> spp. and its toxins are the main contaminants in processing tomato. Based on our earlier research, the current study looked into the anti-fungal capacity of crude lipopeptides from <i>B. amyloliquefaciens</i> XJ-BV2007 against <i>A. alternata</i>. We found that the crude lipopeptides significantly inhibited <i>A. alternata</i> growth and reduced tomato black spot disease incidence. SEM analysis found that the crude lipopeptides could change the morphology of mycelium and spores of <i>A. alternata</i>. Four main <i>Alternaria</i> toxins were detected using UPLC-MS/MS, and the findings demonstrated that the crude lipopeptides could lessen the accumulation of <i>Alternaria</i> toxins in vivo and in vitro. Meanwhile, under the stress of crude lipopeptides, the expression of critical biosynthetic genes responsible for TeA, AOH, and AME was substantially down-regulated. The inhibitory mechanism of the crude lipopeptides was demonstrated to be the disruption of the mycelial structure of <i>A. alternata</i>, as well as the integrity and permeability of the membrane of <i>A. alternata</i> sporocytes. Taken together, crude lipopeptides extracted from <i>B. amyloliquefaciens</i> XJ-BV2007 are an effective biological agent for controlling tomato black spot disease and <i>Alternaria</i> toxins contamination. |
| first_indexed | 2024-03-07T22:11:59Z |
| format | Article |
| id | doaj.art-56692ad2b98c4276994bcf1bdefda09b |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-07T22:11:59Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-56692ad2b98c4276994bcf1bdefda09b2024-02-23T15:36:30ZengMDPI AGToxins2072-66512024-01-011626510.3390/toxins16020065Crude Lipopeptides Produced by <i>Bacillus amyloliquefaciens</i> Could Control the Growth of <i>Alternaria alternata</i> and Production of <i>Alternaria</i> Toxins in Processing TomatoYuanyuan Zhang0Yingying Fan1Yingying Dai2Qinlan Jia3Ying Guo4Peicheng Wang5Tingting Shen6Yan Wang7Fengjuan Liu8Wanhui Guo9Aibo Wu10Ziwei Jiao11Cheng Wang12College of Biological Sciences and Technology, Yili Normal University, Yining 835000, ChinaInstitute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi 830091, ChinaInstitute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi 830091, ChinaInstitute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi 830091, ChinaCollege of Biological Sciences and Technology, Yili Normal University, Yining 835000, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaInstitute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi 830091, ChinaInstitute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi 830091, ChinaInstitute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi 830091, ChinaCAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaCollege of Biological Sciences and Technology, Yili Normal University, Yining 835000, ChinaInstitute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi 830091, China<i>Alternaria</i> spp. and its toxins are the main contaminants in processing tomato. Based on our earlier research, the current study looked into the anti-fungal capacity of crude lipopeptides from <i>B. amyloliquefaciens</i> XJ-BV2007 against <i>A. alternata</i>. We found that the crude lipopeptides significantly inhibited <i>A. alternata</i> growth and reduced tomato black spot disease incidence. SEM analysis found that the crude lipopeptides could change the morphology of mycelium and spores of <i>A. alternata</i>. Four main <i>Alternaria</i> toxins were detected using UPLC-MS/MS, and the findings demonstrated that the crude lipopeptides could lessen the accumulation of <i>Alternaria</i> toxins in vivo and in vitro. Meanwhile, under the stress of crude lipopeptides, the expression of critical biosynthetic genes responsible for TeA, AOH, and AME was substantially down-regulated. The inhibitory mechanism of the crude lipopeptides was demonstrated to be the disruption of the mycelial structure of <i>A. alternata</i>, as well as the integrity and permeability of the membrane of <i>A. alternata</i> sporocytes. Taken together, crude lipopeptides extracted from <i>B. amyloliquefaciens</i> XJ-BV2007 are an effective biological agent for controlling tomato black spot disease and <i>Alternaria</i> toxins contamination.https://www.mdpi.com/2072-6651/16/2/65<i>Bacillus amyloliquefaciens</i>lipopeptide<i>Alternaria alternata</i>processing tomato<i>Alternaria</i> toxins |
| spellingShingle | Yuanyuan Zhang Yingying Fan Yingying Dai Qinlan Jia Ying Guo Peicheng Wang Tingting Shen Yan Wang Fengjuan Liu Wanhui Guo Aibo Wu Ziwei Jiao Cheng Wang Crude Lipopeptides Produced by <i>Bacillus amyloliquefaciens</i> Could Control the Growth of <i>Alternaria alternata</i> and Production of <i>Alternaria</i> Toxins in Processing Tomato Toxins <i>Bacillus amyloliquefaciens</i> lipopeptide <i>Alternaria alternata</i> processing tomato <i>Alternaria</i> toxins |
| title | Crude Lipopeptides Produced by <i>Bacillus amyloliquefaciens</i> Could Control the Growth of <i>Alternaria alternata</i> and Production of <i>Alternaria</i> Toxins in Processing Tomato |
| title_full | Crude Lipopeptides Produced by <i>Bacillus amyloliquefaciens</i> Could Control the Growth of <i>Alternaria alternata</i> and Production of <i>Alternaria</i> Toxins in Processing Tomato |
| title_fullStr | Crude Lipopeptides Produced by <i>Bacillus amyloliquefaciens</i> Could Control the Growth of <i>Alternaria alternata</i> and Production of <i>Alternaria</i> Toxins in Processing Tomato |
| title_full_unstemmed | Crude Lipopeptides Produced by <i>Bacillus amyloliquefaciens</i> Could Control the Growth of <i>Alternaria alternata</i> and Production of <i>Alternaria</i> Toxins in Processing Tomato |
| title_short | Crude Lipopeptides Produced by <i>Bacillus amyloliquefaciens</i> Could Control the Growth of <i>Alternaria alternata</i> and Production of <i>Alternaria</i> Toxins in Processing Tomato |
| title_sort | crude lipopeptides produced by i bacillus amyloliquefaciens i could control the growth of i alternaria alternata i and production of i alternaria i toxins in processing tomato |
| topic | <i>Bacillus amyloliquefaciens</i> lipopeptide <i>Alternaria alternata</i> processing tomato <i>Alternaria</i> toxins |
| url | https://www.mdpi.com/2072-6651/16/2/65 |
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