Tuning recombinant protein expression to match secretion capacity

Tuning recombinant protein expression to match secretion capacity

Abstract Background The secretion of recombinant disulfide-bond containing proteins into the periplasm of Gram-negative bacterial hosts, such as E. coli, has many advantages that can facilitate product isolation, quality and activity. However, the secretion machinery of E. coli has a limited capacit...

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Bibliographic Details
Main Authors: Luminita Gabriela Horga, Samantha Halliwell, Tania Selas Castiñeiras, Chris Wyre, Cristina F. R. O. Matos, Daniela S. Yovcheva, Ross Kent, Rosa Morra, Steven G. Williams, Daniel C. Smith, Neil Dixon
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Microbial Cell Factories
Subjects:
Periplasmic secretion
Antibody fragments
Riboswitches
Codon usage
Signal peptides
Online Access:http://link.springer.com/article/10.1186/s12934-018-1047-z

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http://link.springer.com/article/10.1186/s12934-018-1047-z

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