Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1

Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1

Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA...

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Bibliographic Details
Main Authors: Bo Zhang, Diyin Luo, Yu Li, Vanja Perčulija, Jing Chen, Jinying Lin, Yangmiao Ye, Songying Ouyang
Format: Article
Language:English
Published: Nature Portfolio 2021-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-23876-5

Internet

https://doi.org/10.1038/s41467-021-23876-5

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