Effects of flora deficiency on the structure and function of the large intestine
Summary: The significant anatomical changes in large intestine of germ-free (GF) mice provide excellent material for understanding microbe-host crosstalk. We observed significant differences of GF mice in anatomical and physiological involving in enlarged cecum, thinned mucosal layer and enriched wa...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224001627 |
| _version_ | 1797328593371529216 |
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| author | Tailiang Chai Juan Shen Yifei Sheng Yufen Huang Weiming Liang Zhao Zhang Ruizhen Zhao Haitao Shang Wei Cheng Hang Zhang Xueting Chen Xiang Huang Yin Zhang Jiazhe Liu Huanjie Yang Linying Wang Shanshan Pan Yang Chen Lijuan Han Qinwei Qiu Aibo Gao Hong Wei Xiaodong Fang |
| author_facet | Tailiang Chai Juan Shen Yifei Sheng Yufen Huang Weiming Liang Zhao Zhang Ruizhen Zhao Haitao Shang Wei Cheng Hang Zhang Xueting Chen Xiang Huang Yin Zhang Jiazhe Liu Huanjie Yang Linying Wang Shanshan Pan Yang Chen Lijuan Han Qinwei Qiu Aibo Gao Hong Wei Xiaodong Fang |
| author_sort | Tailiang Chai |
| collection | DOAJ |
| description | Summary: The significant anatomical changes in large intestine of germ-free (GF) mice provide excellent material for understanding microbe-host crosstalk. We observed significant differences of GF mice in anatomical and physiological involving in enlarged cecum, thinned mucosal layer and enriched water in cecal content. Furthermore, integration analysis of multi-omics data revealed the associations between the structure of large intestinal mesenchymal cells and the thinning of the mucosal layer. Increased Aqp8 expression in GF mice may contribute to enhanced water secretion or altered hydrodynamics in the cecum. In addition, the proportion of epithelial cells, nutrient absorption capacity, immune function and the metabolome of cecum contents of large intestine were also significantly altered. Together, this is the first systematic study of the transcriptome and metabolome of the cecum and colon of GF mice, and these findings contribute to our understanding of the intricate interactions between microbes and the large intestine. |
| first_indexed | 2024-03-08T06:53:56Z |
| format | Article |
| id | doaj.art-5699330262234f26ae385f7de3f93693 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T06:53:56Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-5699330262234f26ae385f7de3f936932024-02-03T06:39:05ZengElsevieriScience2589-00422024-02-01272108941Effects of flora deficiency on the structure and function of the large intestineTailiang Chai0Juan Shen1Yifei Sheng2Yufen Huang3Weiming Liang4Zhao Zhang5Ruizhen Zhao6Haitao Shang7Wei Cheng8Hang Zhang9Xueting Chen10Xiang Huang11Yin Zhang12Jiazhe Liu13Huanjie Yang14Linying Wang15Shanshan Pan16Yang Chen17Lijuan Han18Qinwei Qiu19Aibo Gao20Hong Wei21Xiaodong Fang22University of the Chinese Academy of Sciences, College of Life Sciences, Beijing, Beijing, China; BGI, Shenzhen, Guangdong, ChinaBGI, Shenzhen, Guangdong, ChinaUniversity of the Chinese Academy of Sciences, College of Life Sciences, Beijing, Beijing, China; BGI, Shenzhen, Guangdong, ChinaBGI, Shenzhen, Guangdong, ChinaBGI, Shenzhen, Guangdong, ChinaUniversity of the Chinese Academy of Sciences, College of Life Sciences, Beijing, Beijing, China; BGI, Shenzhen, Guangdong, ChinaUniversity of the Chinese Academy of Sciences, College of Life Sciences, Beijing, Beijing, China; BGI, Shenzhen, Guangdong, ChinaSun Yat-sen University First Affiliated Hospital, Precision Medicine Institute, Guangzhou, Guangdong, ChinaHuazhong Agricultural University, College of Animal Sciences and Technology, Wuhan, Hubei, ChinaHuazhong Agricultural University, College of Animal Sciences and Technology, Wuhan, Hubei, ChinaUniversity of the Chinese Academy of Sciences, College of Life Sciences, Beijing, Beijing, China; BGI, Shenzhen, Guangdong, ChinaUniversity of the Chinese Academy of Sciences, College of Life Sciences, Beijing, Beijing, ChinaUniversity of the Chinese Academy of Sciences, College of Life Sciences, Beijing, Beijing, China; BGI, Shenzhen, Guangdong, ChinaBGI, Shenzhen, Guangdong, ChinaBGI, Shenzhen, Guangdong, ChinaBGI, Shenzhen, Guangdong, ChinaBGI-Qingdao, Qingdao, Shandong, ChinaState Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaDepartment of Scientific Research, Kangmeihuada GeneTech Co., Ltd. (KMHD), Shenzhen, ChinaState Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaSun Yat-sen University First Affiliated Hospital, Precision Medicine Institute, Guangzhou, Guangdong, China; Corresponding authorBGI, Shenzhen, Guangdong, China; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China; Corresponding authorSummary: The significant anatomical changes in large intestine of germ-free (GF) mice provide excellent material for understanding microbe-host crosstalk. We observed significant differences of GF mice in anatomical and physiological involving in enlarged cecum, thinned mucosal layer and enriched water in cecal content. Furthermore, integration analysis of multi-omics data revealed the associations between the structure of large intestinal mesenchymal cells and the thinning of the mucosal layer. Increased Aqp8 expression in GF mice may contribute to enhanced water secretion or altered hydrodynamics in the cecum. In addition, the proportion of epithelial cells, nutrient absorption capacity, immune function and the metabolome of cecum contents of large intestine were also significantly altered. Together, this is the first systematic study of the transcriptome and metabolome of the cecum and colon of GF mice, and these findings contribute to our understanding of the intricate interactions between microbes and the large intestine.http://www.sciencedirect.com/science/article/pii/S2589004224001627MicrobiomeMetabolomicsTranscriptomics |
| spellingShingle | Tailiang Chai Juan Shen Yifei Sheng Yufen Huang Weiming Liang Zhao Zhang Ruizhen Zhao Haitao Shang Wei Cheng Hang Zhang Xueting Chen Xiang Huang Yin Zhang Jiazhe Liu Huanjie Yang Linying Wang Shanshan Pan Yang Chen Lijuan Han Qinwei Qiu Aibo Gao Hong Wei Xiaodong Fang Effects of flora deficiency on the structure and function of the large intestine iScience Microbiome Metabolomics Transcriptomics |
| title | Effects of flora deficiency on the structure and function of the large intestine |
| title_full | Effects of flora deficiency on the structure and function of the large intestine |
| title_fullStr | Effects of flora deficiency on the structure and function of the large intestine |
| title_full_unstemmed | Effects of flora deficiency on the structure and function of the large intestine |
| title_short | Effects of flora deficiency on the structure and function of the large intestine |
| title_sort | effects of flora deficiency on the structure and function of the large intestine |
| topic | Microbiome Metabolomics Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224001627 |
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