In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control
Summary: In eukaryotic cells, actin regulates both cytoplasmic and nuclear functions. However, whether actin-based structures are present in the mitochondria and are involved in mitochondrial functions has not been investigated. Here, using wild-type β-actin +/+ and knockout (KO) β-actin −/− mouse e...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-05-01
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| Series: | iScience |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004218300531 |
| _version_ | 1811334063211937792 |
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| author | Xin Xie Tomas Venit Nizar Drou Piergiorgio Percipalle |
| author_facet | Xin Xie Tomas Venit Nizar Drou Piergiorgio Percipalle |
| author_sort | Xin Xie |
| collection | DOAJ |
| description | Summary: In eukaryotic cells, actin regulates both cytoplasmic and nuclear functions. However, whether actin-based structures are present in the mitochondria and are involved in mitochondrial functions has not been investigated. Here, using wild-type β-actin +/+ and knockout (KO) β-actin −/− mouse embryonic fibroblasts we show evidence for the defect in maintaining mitochondrial membrane potential (MMP) in β-actin-null cells. MMP defects were associated with impaired mitochondrial DNA (mtDNA) transcription and nuclear oxidative phosphorylation (OXPHOS) gene expression. Using super-resolution microscopy we provided direct evidence on the presence of β-actin-containing structures inside mitochondria. Large aggregates of TFAM-stained nucleoids were observed in bulb-shaped mitochondria in KO cells, suggesting defects in mitochondrial nucleoid segregation without β-actin. The observation that mitochondria-targeted β-actin rescued mtDNA transcription and MMP suggests an indispensable functional role of a mitochondrial β-actin pool necessary for mitochondrial quality control. : Molecular Biology; Cell Biology; Functional Aspects of Cell Biology Subject Areas: Molecular Biology, Cell Biology, Functional Aspects of Cell Biology |
| first_indexed | 2024-04-13T17:02:12Z |
| format | Article |
| id | doaj.art-56a7e5b914a1465e87eb2100403ccb0d |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-13T17:02:12Z |
| publishDate | 2018-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-56a7e5b914a1465e87eb2100403ccb0d2022-12-22T02:38:36ZengElsevieriScience2589-00422018-05-013226237In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality ControlXin Xie0Tomas Venit1Nizar Drou2Piergiorgio Percipalle3Science Division, Biology Program, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, United Arab EmiratesScience Division, Biology Program, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, United Arab EmiratesNYU Abu Dhabi Center for Genomics and Systems Biology, Abu Dhabi, UAEScience Division, Biology Program, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, United Arab Emirates; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, S-106 91 Stockholm, Sweden; Corresponding authorSummary: In eukaryotic cells, actin regulates both cytoplasmic and nuclear functions. However, whether actin-based structures are present in the mitochondria and are involved in mitochondrial functions has not been investigated. Here, using wild-type β-actin +/+ and knockout (KO) β-actin −/− mouse embryonic fibroblasts we show evidence for the defect in maintaining mitochondrial membrane potential (MMP) in β-actin-null cells. MMP defects were associated with impaired mitochondrial DNA (mtDNA) transcription and nuclear oxidative phosphorylation (OXPHOS) gene expression. Using super-resolution microscopy we provided direct evidence on the presence of β-actin-containing structures inside mitochondria. Large aggregates of TFAM-stained nucleoids were observed in bulb-shaped mitochondria in KO cells, suggesting defects in mitochondrial nucleoid segregation without β-actin. The observation that mitochondria-targeted β-actin rescued mtDNA transcription and MMP suggests an indispensable functional role of a mitochondrial β-actin pool necessary for mitochondrial quality control. : Molecular Biology; Cell Biology; Functional Aspects of Cell Biology Subject Areas: Molecular Biology, Cell Biology, Functional Aspects of Cell Biologyhttp://www.sciencedirect.com/science/article/pii/S2589004218300531 |
| spellingShingle | Xin Xie Tomas Venit Nizar Drou Piergiorgio Percipalle In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control iScience |
| title | In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control |
| title_full | In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control |
| title_fullStr | In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control |
| title_full_unstemmed | In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control |
| title_short | In Mitochondria β-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control |
| title_sort | in mitochondria β actin regulates mtdna transcription and is required for mitochondrial quality control |
| url | http://www.sciencedirect.com/science/article/pii/S2589004218300531 |
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