Large-scale RT-PCR recovery of full-length cDNA clones

Large-scale RT-PCR recovery of full-length cDNA clones

Pseudogenes, alternative transcripts, noncoding RNA, and polymorphisms each add extensive complexity to the mammalian transcriptome and confound estimation of the total number of genes. Despite advanced algorithms for gene prediction and several large-scale efforts to obtain cDNA clones for all huma...

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Bibliographic Details
Main Authors: Jia Qian Wu, Angela M. Garcia, Steven Hulyk, Anna Sneed, Carla Kowis, Ye Yuan, David Steffen, John D. McPherson, Preethi H. Gunaratne, Richard A. Gibbs
Format: Article
Language:English
Published: Taylor & Francis Group 2004-04-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/04364DD03

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https://www.future-science.com/doi/10.2144/04364DD03

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