Heterologous Immune Responses of Serum IgG and Secretory IgA Against the Spike Protein of Endemic Coronaviruses During Severe COVID-19
Defining immune correlates of disease severity is important to better understand the immunopathogenesis in COVID-19. Here we made use of a protein microarray platform to detect IgG- and IgA-reactive antibodies in sera and saliva respectively, and assess cross-reactivity between SARS-CoV-2 and endemi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.839367/full |
| _version_ | 1819122326024749056 |
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| author | Wouter L. Smit Wouter L. Smit Sophie van Tol Sanne van der Wal Femke van Vulpen Shannon la Grouw Lenneke van Lelyveld Gijs Limonard Ailko Bossink Gert-Jan Godeke Sandhya Shrestha Johan Reimerink Dirk Eggink Chantal Reusken Michiel Heron Steven Thijsen |
| author_facet | Wouter L. Smit Wouter L. Smit Sophie van Tol Sanne van der Wal Femke van Vulpen Shannon la Grouw Lenneke van Lelyveld Gijs Limonard Ailko Bossink Gert-Jan Godeke Sandhya Shrestha Johan Reimerink Dirk Eggink Chantal Reusken Michiel Heron Steven Thijsen |
| author_sort | Wouter L. Smit |
| collection | DOAJ |
| description | Defining immune correlates of disease severity is important to better understand the immunopathogenesis in COVID-19. Here we made use of a protein microarray platform to detect IgG- and IgA-reactive antibodies in sera and saliva respectively, and assess cross-reactivity between SARS-CoV-2 and endemic coronaviruses (eCoVs). IgG responses against the full protein of spike, but not the S1 subunit, were significantly higher in convalescent sera of patients with severe disease compared to mild disease and healthy controls. In addition, we detected reactivity of secretory IgA to eCoVs in saliva of patients with severe disease, not present in patients with moderate disease or seropositive healthy controls. These heterologous immune responses are in line with non-protective cross-reactivity, and support a potential role for immune imprinting in the pathogenesis of severe COVID-19. |
| first_indexed | 2024-12-22T06:50:40Z |
| format | Article |
| id | doaj.art-56c6023616cd4ffdb9c836ce5a805f9e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T06:50:40Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-56c6023616cd4ffdb9c836ce5a805f9e2022-12-21T18:35:09ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-03-011310.3389/fimmu.2022.839367839367Heterologous Immune Responses of Serum IgG and Secretory IgA Against the Spike Protein of Endemic Coronaviruses During Severe COVID-19Wouter L. Smit0Wouter L. Smit1Sophie van Tol2Sanne van der Wal3Femke van Vulpen4Shannon la Grouw5Lenneke van Lelyveld6Gijs Limonard7Ailko Bossink8Gert-Jan Godeke9Sandhya Shrestha10Johan Reimerink11Dirk Eggink12Chantal Reusken13Michiel Heron14Steven Thijsen15Department of Medical Microbiology and Immunology, Diakonessenhuis Utrecht, Utrecht, NetherlandsDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, NetherlandsCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, NetherlandsDepartment of Medical Microbiology and Immunology, Diakonessenhuis Utrecht, Utrecht, NetherlandsDepartment of Medical Microbiology and Immunology, Diakonessenhuis Utrecht, Utrecht, NetherlandsDepartment of Medical Microbiology and Immunology, Diakonessenhuis Utrecht, Utrecht, NetherlandsDepartment of Intensive Care, Diakonessenhuis Utrecht, Utrecht, NetherlandsDepartment of Pulmonary Diseases, Diakonessenhuis Utrecht, Utrecht, NetherlandsDepartment of Pulmonary Diseases, Diakonessenhuis Utrecht, Utrecht, NetherlandsCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, NetherlandsCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, NetherlandsCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, NetherlandsCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, NetherlandsCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, NetherlandsDepartment of Medical Microbiology and Immunology, Diakonessenhuis Utrecht, Utrecht, NetherlandsDepartment of Medical Microbiology and Immunology, Diakonessenhuis Utrecht, Utrecht, NetherlandsDefining immune correlates of disease severity is important to better understand the immunopathogenesis in COVID-19. Here we made use of a protein microarray platform to detect IgG- and IgA-reactive antibodies in sera and saliva respectively, and assess cross-reactivity between SARS-CoV-2 and endemic coronaviruses (eCoVs). IgG responses against the full protein of spike, but not the S1 subunit, were significantly higher in convalescent sera of patients with severe disease compared to mild disease and healthy controls. In addition, we detected reactivity of secretory IgA to eCoVs in saliva of patients with severe disease, not present in patients with moderate disease or seropositive healthy controls. These heterologous immune responses are in line with non-protective cross-reactivity, and support a potential role for immune imprinting in the pathogenesis of severe COVID-19.https://www.frontiersin.org/articles/10.3389/fimmu.2022.839367/fullSARS-CoV-2COVID-19spike proteinseasonal coronavirusesimmune imprinting |
| spellingShingle | Wouter L. Smit Wouter L. Smit Sophie van Tol Sanne van der Wal Femke van Vulpen Shannon la Grouw Lenneke van Lelyveld Gijs Limonard Ailko Bossink Gert-Jan Godeke Sandhya Shrestha Johan Reimerink Dirk Eggink Chantal Reusken Michiel Heron Steven Thijsen Heterologous Immune Responses of Serum IgG and Secretory IgA Against the Spike Protein of Endemic Coronaviruses During Severe COVID-19 Frontiers in Immunology SARS-CoV-2 COVID-19 spike protein seasonal coronaviruses immune imprinting |
| title | Heterologous Immune Responses of Serum IgG and Secretory IgA Against the Spike Protein of Endemic Coronaviruses During Severe COVID-19 |
| title_full | Heterologous Immune Responses of Serum IgG and Secretory IgA Against the Spike Protein of Endemic Coronaviruses During Severe COVID-19 |
| title_fullStr | Heterologous Immune Responses of Serum IgG and Secretory IgA Against the Spike Protein of Endemic Coronaviruses During Severe COVID-19 |
| title_full_unstemmed | Heterologous Immune Responses of Serum IgG and Secretory IgA Against the Spike Protein of Endemic Coronaviruses During Severe COVID-19 |
| title_short | Heterologous Immune Responses of Serum IgG and Secretory IgA Against the Spike Protein of Endemic Coronaviruses During Severe COVID-19 |
| title_sort | heterologous immune responses of serum igg and secretory iga against the spike protein of endemic coronaviruses during severe covid 19 |
| topic | SARS-CoV-2 COVID-19 spike protein seasonal coronaviruses immune imprinting |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.839367/full |
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