Protocol to evaluate cell lineage stability of mouse natural and induced regulatory T cells using bisulfite sequencing

Protocol to evaluate cell lineage stability of mouse natural and induced regulatory T cells using bisulfite sequencing

Summary: The establishment of regulatory T cells (Treg)-specific demethylation regions (TSDRs) is essential for the Treg-lineage stability. Here, we present a protocol using bisulfite sequencing to assess Treg-lineage stability. The protocol describes the isolation of lymphocytes and DNA extraction,...

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Bibliographic Details
Main Authors: Masaya Arai, Aine Fukuda, Reo Morimoto, Yamami Nakamura, Zhaohong Ci, Shimon Sakaguchi
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell biology
Cell isolation
Flow cytometry/Mass cytometry
Genetics
Sequencing
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005743

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http://www.sciencedirect.com/science/article/pii/S2666166722005743

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