Procedure of the overexpression, purification and crystallization of BLEG-1, a bifunctional and evolutionary divergent B3 metallo-β-lactamase, for structure-function studies

Procedure of the overexpression, purification and crystallization of BLEG-1, a bifunctional and evolutionary divergent B3 metallo-β-lactamase, for structure-function studies

Metallo-β-lactamases (MBLs) are class B β-lactamases from the metallo-hydrolase-like MBL-fold superfamily which act on a broad range of β-lactam antibiotics, thus conferring antibiotics resistance to bacterial pathogens. The attempt to structurally characterize BLEG-1, an evolutionary divergent B3 m...

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Bibliographic Details
Main Authors: Shaw Xian Au, Noor Dina Muhd Noor, Hiroyoshi Matsumura, Raja Noor Zaliha Raja Abdul Rahman, Yahaya M. Normi
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:MethodsX
Subjects:
BLEG-1
B3 metallo-β-lactamase
Protein production
Protein purification
Protein crystallography
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016122001212

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http://www.sciencedirect.com/science/article/pii/S2215016122001212

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