Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays

Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays

T cell monitoring is increasingly performed using cryopreserved PBMC. It has been suggested that resting of PBMC after thawing, that is, culturing them overnight in test medium, produces higher antigen-induced spot counts in ELISPOT assays. To evaluate the importance of overnight resting, we systema...

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Bibliographic Details
Main Authors: Ramu A. Subbramanian, Wenji Zhang, Edith Karacsony, Mascha S. Recks, Helena Batoulis, Stefanie Kuerten, Paul V. Lehmann
Format: Article
Language:English
Published: MDPI AG 2012-07-01
Series:Cells
Subjects:
cryopreservation
CEF
high-throughput
mumps
T cells
Online Access:http://www.mdpi.com/2073-4409/1/3/409

Internet

http://www.mdpi.com/2073-4409/1/3/409

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