Protocol for preparing formalin-fixed paraffin-embedded musculoskeletal tissue samples from mice for spatial transcriptomics

Protocol for preparing formalin-fixed paraffin-embedded musculoskeletal tissue samples from mice for spatial transcriptomics

Summary: Here, we present a protocol for using spatial transcriptomics in bone and multi-tissue musculoskeletal formalin-fixed paraffin-embedded (FFPE) samples from mice. We describe steps for tissue harvesting, sample preparation, paraffin embedding, and FFPE sample selection. We detail procedures...

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Bibliographic Details
Main Authors: Esther Wehrle, Denise Günther, Neashan Mathavan, Amit Singh, Ralph Müller
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Health Sciences
Genomics
Microscopy
Molecular Biology
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724001515

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http://www.sciencedirect.com/science/article/pii/S2666166724001515

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