Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI

<p>Abstract</p> <p>Background</p> <p>The basophil activation test (BAT), in which translocation of markers to the surface of blood basophils is measured in response to allergen by flow cytometry, is a rapid assay that is gaining popularity. Two markers are currently bei...

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Bibliographic Details
Main Authors: Nielsen Lars, Bøgebjerg Mette, Hoffmann Hans Jürgen, Dahl Ronald
Format: Article
Language:English
Published: BMC 2005-07-01
Series:Clinical and Molecular Allergy
Online Access:http://www.clinicalmolecularallergy.com/content/3/1/10