Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells
HIV transmission via genital and colorectal mucosa are the most common routes of dissemination. Here, we explored the effects of free and complement-opsonized HIV on colorectal tissue. Initially, there was higher antiviral responses in the free HIV compared to complement-opsonized virus. The mucosal...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-09-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/57869 |
| _version_ | 1797997617878138880 |
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| author | Pradyot Bhattacharya Rada Ellegård Mohammad Khalid Cecilia Svanberg Melissa Govender Åsa V Keita Johan D Söderholm Pär Myrelid Esaki M Shankar Sofia Nyström Marie Larsson |
| author_facet | Pradyot Bhattacharya Rada Ellegård Mohammad Khalid Cecilia Svanberg Melissa Govender Åsa V Keita Johan D Söderholm Pär Myrelid Esaki M Shankar Sofia Nyström Marie Larsson |
| author_sort | Pradyot Bhattacharya |
| collection | DOAJ |
| description | HIV transmission via genital and colorectal mucosa are the most common routes of dissemination. Here, we explored the effects of free and complement-opsonized HIV on colorectal tissue. Initially, there was higher antiviral responses in the free HIV compared to complement-opsonized virus. The mucosal transcriptional response at 24 hr revealed the involvement of activated T cells, which was mirrored in cellular responses observed at 96 hr in isolated mucosal T cells. Further, HIV exposure led to skewing of T cell phenotypes predominantly to inflammatory CD4+ T cells, that is Th17 and Th1Th17 subsets. Of note, HIV exposure created an environment that altered the CD8+ T cell phenotype, for example expression of regulatory factors, especially when the virions were opsonized with complement factors. Our findings suggest that HIV-opsonization alters the activation and signaling pathways in the colorectal mucosa, which promotes viral establishment by creating an environment that stimulates mucosal T cell activation and inflammatory Th cells. |
| first_indexed | 2024-04-11T10:35:38Z |
| format | Article |
| id | doaj.art-5784e462455c4401acbb84dc90a711e0 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:35:38Z |
| publishDate | 2020-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5784e462455c4401acbb84dc90a711e02022-12-22T04:29:18ZengeLife Sciences Publications LtdeLife2050-084X2020-09-01910.7554/eLife.57869Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cellsPradyot Bhattacharya0Rada Ellegård1Mohammad Khalid2Cecilia Svanberg3Melissa Govender4Åsa V Keita5Johan D Söderholm6Pär Myrelid7Esaki M Shankar8Sofia Nyström9Marie Larsson10https://orcid.org/0000-0002-4524-0177Division of Molecular Medicine and Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, SwedenDivision of Molecular Medicine and Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, SwedenDivision of Molecular Medicine and Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, SwedenDivision of Molecular Medicine and Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, SwedenDivision of Molecular Medicine and Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, SwedenDivision of Surgery, Orthopedics and Oncology, Linköping University, Linköping, SwedenDivision of Surgery, Orthopedics and Oncology, Linköping University, Linköping, SwedenDivision of Surgery, Orthopedics and Oncology, Linköping University, Linköping, SwedenCenter of Excellence for Research in AIDS (CERiA), University of Malaya, Lembah Pantai, Kuala Lumpur, Malaysia; Division of Infection Biology and Medical Microbiology, Department of Life Sciences, Central University of Tamil Nadu, Thiruvarur, IndiaDivision of Molecular Medicine and Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Department of Clinical Immunology and Transfusion Medicine and Department of Clinical and Experimental Medicine, Linköping University, Linköping, SwedenDivision of Molecular Medicine and Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, SwedenHIV transmission via genital and colorectal mucosa are the most common routes of dissemination. Here, we explored the effects of free and complement-opsonized HIV on colorectal tissue. Initially, there was higher antiviral responses in the free HIV compared to complement-opsonized virus. The mucosal transcriptional response at 24 hr revealed the involvement of activated T cells, which was mirrored in cellular responses observed at 96 hr in isolated mucosal T cells. Further, HIV exposure led to skewing of T cell phenotypes predominantly to inflammatory CD4+ T cells, that is Th17 and Th1Th17 subsets. Of note, HIV exposure created an environment that altered the CD8+ T cell phenotype, for example expression of regulatory factors, especially when the virions were opsonized with complement factors. Our findings suggest that HIV-opsonization alters the activation and signaling pathways in the colorectal mucosa, which promotes viral establishment by creating an environment that stimulates mucosal T cell activation and inflammatory Th cells.https://elifesciences.org/articles/57869HIVcolorectal mucosavirus infectiont lymphocytesinnnate responses |
| spellingShingle | Pradyot Bhattacharya Rada Ellegård Mohammad Khalid Cecilia Svanberg Melissa Govender Åsa V Keita Johan D Söderholm Pär Myrelid Esaki M Shankar Sofia Nyström Marie Larsson Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells eLife HIV colorectal mucosa virus infection t lymphocytes innnate responses |
| title | Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells |
| title_full | Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells |
| title_fullStr | Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells |
| title_full_unstemmed | Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells |
| title_short | Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells |
| title_sort | complement opsonization of hiv affects primary infection of human colorectal mucosa and subsequent activation of t cells |
| topic | HIV colorectal mucosa virus infection t lymphocytes innnate responses |
| url | https://elifesciences.org/articles/57869 |
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