Rapid and Sensitive Detection of Tomato Brown Rugose Fruit Virus in Tomato and Pepper Seeds by Reverse Transcription Loop-Mediated Isothermal Amplification Assays (Real Time and Visual) and Comparison With RT-PCR End-Point and RT-qPCR Methods

Rapid and Sensitive Detection of Tomato Brown Rugose Fruit Virus in Tomato and Pepper Seeds by Reverse Transcription Loop-Mediated Isothermal Amplification Assays (Real Time and Visual) and Comparison With RT-PCR End-Point and RT-qPCR Methods

Tomato brown rugose fruit virus (ToBRFV) represents an emerging viral threat to the productivity of tomato and pepper protected cultivation worldwide. This virus has got the status of quarantine organism in the European Union (EU) countries. In particular, tomato and pepper seeds will need to be fre...

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Bibliographic Details
Main Authors: Domenico Rizzo, Daniele Da Lio, Alessandra Panattoni, Chiara Salemi, Giovanni Cappellini, Linda Bartolini, Giuseppe Parrella
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Microbiology
Subjects:
virus diagnostic
seeds contamination
seed testing
tomato brown rugose fruit virus
RT-LAMP assay
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.640932/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.640932/full

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