Duplex One-Step RT-qPCR Assays for Simultaneous Detection of Genomic and Subgenomic RNAs of SARS-CoV-2 Variants

Duplex One-Step RT-qPCR Assays for Simultaneous Detection of Genomic and Subgenomic RNAs of SARS-CoV-2 Variants

A hallmark of severe acute respiratory syndrome virus (SARS-CoV-2) replication is the discontinuous transcription of open reading frames (ORFs) encoding structural virus proteins. Real-time reverse transcription PCR (RT-qPCR) assays in previous publications used either single or multiplex assays for...

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Bibliographic Details
Main Authors: Sushma M. Bhosle, Julie P. Tran, Shuiqing Yu, Jillian Geiger, Jennifer D. Jackson, Ian Crozier, Anya Crane, Jiro Wada, Travis K. Warren, Jens H. Kuhn, Gabriella Worwa
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Viruses
Subjects:
SARS-CoV-2
variant
duplex RT-qPCR
RNA
genomic
subgenomic
Online Access:https://www.mdpi.com/1999-4915/14/5/1066

Internet

https://www.mdpi.com/1999-4915/14/5/1066

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