Flow cytometry analysis of endothelial cells and subsets of exhausted CD8+ T cells in murine tumor models

Flow cytometry analysis of endothelial cells and subsets of exhausted CD8+ T cells in murine tumor models

Summary: Here, we present a protocol for flow cytometry analysis of endothelial cells (ECs) and CD8+ T cells in murine tumor models, at baseline and after cancer immunotherapy with anti-PD-1/anti-CTLA-4 antibodies. We provide gating strategies for identification of specific cell subsets including EC...

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Bibliographic Details
Main Authors: Lucas Blanchard, Estefania Vina, Assia Asrir, Claire Tardiveau, Juliette Coudert, Robin Laffont, Dorian Tarroux, Sarah Bettini, Krystle Veerman, Fanny Lafouresse, Mélanie Pichery, Emilie Mirey, Elisabeth Bellard, Nathalie Ortega, Jean-Philippe Girard
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
Cancer
Health Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003240

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http://www.sciencedirect.com/science/article/pii/S2666166722003240

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