High-yield expression of heterologous [FeFe] hydrogenases in Escherichia coli.

BACKGROUND: The realization of hydrogenase-based technologies for renewable H(2) production is presently limited by the need for scalable and high-yielding methods to supply active hydrogenases and their required maturases. PRINCIPAL FINDINGS: In this report, we describe an improved Escherichia coli...

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Bibliographic Details
Main Authors: Jon M Kuchenreuther, Celestine S Grady-Smith, Alyssa S Bingham, Simon J George, Stephen P Cramer, James R Swartz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2991362?pdf=render