Validation of RNA Extraction Methods and Suitable Reference Genes for Gene Expression Studies in Developing Fetal Human Inner Ear Tissue

Validation of RNA Extraction Methods and Suitable Reference Genes for Gene Expression Studies in Developing Fetal Human Inner Ear Tissue

A comprehensive gene expression investigation requires high-quality RNA extraction, in sufficient amounts for real-time quantitative polymerase chain reaction and next-generation sequencing. In this work, we compared different RNA extraction methods and evaluated different reference genes for gene e...

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Bibliographic Details
Main Authors: Claudia Steinacher, Dietmar Rieder, Jasmin E. Turner, Nita Solanky, Shin-ya Nishio, Shin-ichi Usami, Barbara Hausott, Anneliese Schrott-Fischer, Jozsef Dudas
Format: Article
Language:English
Published: MDPI AG 2024-03-01
Series:International Journal of Molecular Sciences
Subjects:
human fetal inner ear
reference gene
RNA extraction
RNA expression
<i>TECTA</i>
<i>OTOF</i>
Online Access:https://www.mdpi.com/1422-0067/25/5/2907

Internet

https://www.mdpi.com/1422-0067/25/5/2907

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