CD40 ligand stimulation affects the number and memory phenotypes of human peripheral CD8+ T cells
Abstract Background CD40L is primarily expressed on activated CD4+ T cells and binds to CD40 which is expressed by various cells including dendritic cells, macrophages and B lymphocytes. While CD40-CD40L interaction is known to be direct between B cells and CD4+ T cells which results in proliferatio...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Immunology |
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| Online Access: | https://doi.org/10.1186/s12865-023-00547-2 |
| _version_ | 1797789843734921216 |
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| author | Haeyoun Choi Hyun-Joo Lee Hyun-Jung Sohn Tai-Gyu Kim |
| author_facet | Haeyoun Choi Hyun-Joo Lee Hyun-Jung Sohn Tai-Gyu Kim |
| author_sort | Haeyoun Choi |
| collection | DOAJ |
| description | Abstract Background CD40L is primarily expressed on activated CD4+ T cells and binds to CD40 which is expressed by various cells including dendritic cells, macrophages and B lymphocytes. While CD40-CD40L interaction is known to be direct between B cells and CD4+ T cells which results in proliferation and immunoglobulin isotype switching, antigen presenting cells (APCs) were thought to be involved in the delivery of CD4+ help to CD8+ T cells by cross-talk between CD4+ and CD8+ T cells and APCs. However, subsequent study demonstrated that CD40L signal can be directly delivered to CD8+ T cells by CD40 expression on CD8+ T cells. Since most studies have been carried out in murine models, we aimed to investigate the direct effect of CD40L on human peripheral CD8+ T cells. Results Human peripheral CD8+ T cells were isolated to exclude the indirect effect of B cells or dendritic cells. Upon activation, CD40 expression on CD8+ T cells was transiently induced and stimulation with artificial APCs expressing CD40L (aAPC-CD40L) increased the number of total and central memory CD8+ T cells and also pp65 specific CD8+ T cells. Stimulation with aAPC-CD40L also resulted in higher proportion of central memory CD8+ T cells. Conclusions Our study suggests that CD40L has an effect on the increased number of CD8+ T cells through CD40 expressed on activated CD8+ T cells and has influence on memory CD8+ T cell generation. Our results may provide a new perspective of the effect of CD40L on human peripheral CD8+ T cells, which differ according to the memory differentiation status of CD8+ T cells. |
| first_indexed | 2024-03-13T01:56:18Z |
| format | Article |
| id | doaj.art-57d4a3cbb3134f21a70902494cf55c4b |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-03-13T01:56:18Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-57d4a3cbb3134f21a70902494cf55c4b2023-07-02T11:11:51ZengBMCBMC Immunology1471-21722023-06-012411910.1186/s12865-023-00547-2CD40 ligand stimulation affects the number and memory phenotypes of human peripheral CD8+ T cellsHaeyoun Choi0Hyun-Joo Lee1Hyun-Jung Sohn2Tai-Gyu Kim3Department of Microbiology, College of Medicine, The Catholic University of KoreaCatholic Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of KoreaCatholic Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of KoreaDepartment of Microbiology, College of Medicine, The Catholic University of KoreaAbstract Background CD40L is primarily expressed on activated CD4+ T cells and binds to CD40 which is expressed by various cells including dendritic cells, macrophages and B lymphocytes. While CD40-CD40L interaction is known to be direct between B cells and CD4+ T cells which results in proliferation and immunoglobulin isotype switching, antigen presenting cells (APCs) were thought to be involved in the delivery of CD4+ help to CD8+ T cells by cross-talk between CD4+ and CD8+ T cells and APCs. However, subsequent study demonstrated that CD40L signal can be directly delivered to CD8+ T cells by CD40 expression on CD8+ T cells. Since most studies have been carried out in murine models, we aimed to investigate the direct effect of CD40L on human peripheral CD8+ T cells. Results Human peripheral CD8+ T cells were isolated to exclude the indirect effect of B cells or dendritic cells. Upon activation, CD40 expression on CD8+ T cells was transiently induced and stimulation with artificial APCs expressing CD40L (aAPC-CD40L) increased the number of total and central memory CD8+ T cells and also pp65 specific CD8+ T cells. Stimulation with aAPC-CD40L also resulted in higher proportion of central memory CD8+ T cells. Conclusions Our study suggests that CD40L has an effect on the increased number of CD8+ T cells through CD40 expressed on activated CD8+ T cells and has influence on memory CD8+ T cell generation. Our results may provide a new perspective of the effect of CD40L on human peripheral CD8+ T cells, which differ according to the memory differentiation status of CD8+ T cells.https://doi.org/10.1186/s12865-023-00547-2CD40LCD8+ T cellProliferationMemoryArtificial antigen presenting cell |
| spellingShingle | Haeyoun Choi Hyun-Joo Lee Hyun-Jung Sohn Tai-Gyu Kim CD40 ligand stimulation affects the number and memory phenotypes of human peripheral CD8+ T cells BMC Immunology CD40L CD8+ T cell Proliferation Memory Artificial antigen presenting cell |
| title | CD40 ligand stimulation affects the number and memory phenotypes of human peripheral CD8+ T cells |
| title_full | CD40 ligand stimulation affects the number and memory phenotypes of human peripheral CD8+ T cells |
| title_fullStr | CD40 ligand stimulation affects the number and memory phenotypes of human peripheral CD8+ T cells |
| title_full_unstemmed | CD40 ligand stimulation affects the number and memory phenotypes of human peripheral CD8+ T cells |
| title_short | CD40 ligand stimulation affects the number and memory phenotypes of human peripheral CD8+ T cells |
| title_sort | cd40 ligand stimulation affects the number and memory phenotypes of human peripheral cd8 t cells |
| topic | CD40L CD8+ T cell Proliferation Memory Artificial antigen presenting cell |
| url | https://doi.org/10.1186/s12865-023-00547-2 |
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