Imaging the early zebrafish embryo centrosomes following injection of small-molecule inhibitors to understand spindle formation

Summary: During the earliest division stages, zebrafish embryos have large cells that divide rapidly and synchronously to create a cellular layer on top of the yolk. Here, we describe a protocol for monitoring spindle dynamics during these early embryonic divisions. We outline techniques for injecti...

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Bibliographic Details
Main Authors: Abrar A. Aljiboury, Amra Mujcic, Thomas Cammerino, Lindsay I. Rathbun, Heidi Hehnly
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672030280X