Imaging the early zebrafish embryo centrosomes following injection of small-molecule inhibitors to understand spindle formation
Summary: During the earliest division stages, zebrafish embryos have large cells that divide rapidly and synchronously to create a cellular layer on top of the yolk. Here, we describe a protocol for monitoring spindle dynamics during these early embryonic divisions. We outline techniques for injecti...
Main Authors: | Abrar A. Aljiboury, Amra Mujcic, Thomas Cammerino, Lindsay I. Rathbun, Heidi Hehnly |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2021-03-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672030280X |
Similar Items
-
Akt regulates centrosome migration and spindle orientation in the early Drosophila melanogaster embryo.
by: Buttrick, G, et al.
Published: (2008) -
Expansion microscopy of the chick embryo neural tube to overcome molecular crowding at the centrosomes-cilia
by: Axelle Wilmerding, et al.
Published: (2023-03-01) -
Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
by: Beatriz A. Ibarra, et al.
Published: (2022-06-01) -
Separate To Operate: the Centriole-Free Inner Core of the Centrosome Regulates the Assembly of the Intranuclear Spindle in Toxoplasma gondii
by: Ramiro Tomasina, et al.
Published: (2022-10-01) -
Using single-molecule fluorescence in situ hybridization and immunohistochemistry to count RNA molecules in single cells in zebrafish embryos
by: Kemal Keseroglu, et al.
Published: (2023-03-01)