Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf/MEK/ERK signaling pathway
Objective To explore the effect of renal sympathetic denervation (RSD) on left ventricle hypertrophy and the Raf/MEK/ERK signaling pathway in spontaneously hypertensive rats (SHRs). Methods SHRs were divided into SHR, SHR + Sham, SHR + RSD and SHR + U0126 groups, with WKY rats as the baseline contro...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-02-01
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| Series: | Clinical and Experimental Hypertension |
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| Online Access: | http://dx.doi.org/10.1080/10641963.2020.1833022 |
| _version_ | 1797681293037666304 |
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| author | Bing Xiao Fan Liu Ye-Hui Jin Ya-Qiong Jin Li Wang Jing-Chao Lu Xiu-Chun Yang |
| author_facet | Bing Xiao Fan Liu Ye-Hui Jin Ya-Qiong Jin Li Wang Jing-Chao Lu Xiu-Chun Yang |
| author_sort | Bing Xiao |
| collection | DOAJ |
| description | Objective To explore the effect of renal sympathetic denervation (RSD) on left ventricle hypertrophy and the Raf/MEK/ERK signaling pathway in spontaneously hypertensive rats (SHRs). Methods SHRs were divided into SHR, SHR + Sham, SHR + RSD and SHR + U0126 groups, with WKY rats as the baseline controls. The blood pressure of rats was observed, while myocardial fibrosis was evaluated through Masson staining. Thereafter, real-time quantitative polymerase chain reaction (qRT-PCR) was carried out to determine the levels of myocardial-hypertrophy-related markers, and Western blotting was used to measure the activity of the Raf/MEK/ERK signaling pathway. Results In comparison with the WKY group, significant increases were observed in the systolic pressure and diastolic pressure of rats from the other four groups at different time points after surgery. In addition, rats in these groups had obvious increases in LVMI, renal NE and IVSd and decreases in LVEDd, LVEF and LVFS. In addition, the CVF of myocardial tissues was increased, with the upregulation of ANP, BNP and β-MHC and the downregulation of α-MHC. For the activity of the Raf/MEK/ERK signaling pathway, the levels of p-Raf/Raf, p-MEK/MEK and p-ERK1/2/ERK1/2 were all remarkably elevated (all P < .05). Further comparison with the SHR group showed that the above indexes in the rats were significantly improved in the RSD group and SHR + U0126 group (all P < .05). Conclusion RSD may decrease blood pressure, mitigate hypertension-induced left ventricle hypertrophy and improve cardiac function efficiently in SHRs via the suppression of the Raf/MEK/ERK signaling pathway. |
| first_indexed | 2024-03-11T23:42:52Z |
| format | Article |
| id | doaj.art-57d66dad41484e7f90d0f7f1d9e2c705 |
| institution | Directory Open Access Journal |
| issn | 1064-1963 1525-6006 |
| language | English |
| last_indexed | 2024-03-11T23:42:52Z |
| publishDate | 2021-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Clinical and Experimental Hypertension |
| spelling | doaj.art-57d66dad41484e7f90d0f7f1d9e2c7052023-09-19T15:19:29ZengTaylor & Francis GroupClinical and Experimental Hypertension1064-19631525-60062021-02-0143214215010.1080/10641963.2020.18330221833022Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf/MEK/ERK signaling pathwayBing Xiao0Fan Liu1Ye-Hui Jin2Ya-Qiong Jin3Li Wang4Jing-Chao Lu5Xiu-Chun Yang6The Second Hospital of Hebei Medical UniversityThe Second Hospital of Hebei Medical UniversityThe Second Hospital of Hebei Medical UniversityThe Second Hospital of Hebei Medical UniversityThe Second Hospital of Hebei Medical UniversityThe Second Hospital of Hebei Medical UniversityThe Second Hospital of Hebei Medical UniversityObjective To explore the effect of renal sympathetic denervation (RSD) on left ventricle hypertrophy and the Raf/MEK/ERK signaling pathway in spontaneously hypertensive rats (SHRs). Methods SHRs were divided into SHR, SHR + Sham, SHR + RSD and SHR + U0126 groups, with WKY rats as the baseline controls. The blood pressure of rats was observed, while myocardial fibrosis was evaluated through Masson staining. Thereafter, real-time quantitative polymerase chain reaction (qRT-PCR) was carried out to determine the levels of myocardial-hypertrophy-related markers, and Western blotting was used to measure the activity of the Raf/MEK/ERK signaling pathway. Results In comparison with the WKY group, significant increases were observed in the systolic pressure and diastolic pressure of rats from the other four groups at different time points after surgery. In addition, rats in these groups had obvious increases in LVMI, renal NE and IVSd and decreases in LVEDd, LVEF and LVFS. In addition, the CVF of myocardial tissues was increased, with the upregulation of ANP, BNP and β-MHC and the downregulation of α-MHC. For the activity of the Raf/MEK/ERK signaling pathway, the levels of p-Raf/Raf, p-MEK/MEK and p-ERK1/2/ERK1/2 were all remarkably elevated (all P < .05). Further comparison with the SHR group showed that the above indexes in the rats were significantly improved in the RSD group and SHR + U0126 group (all P < .05). Conclusion RSD may decrease blood pressure, mitigate hypertension-induced left ventricle hypertrophy and improve cardiac function efficiently in SHRs via the suppression of the Raf/MEK/ERK signaling pathway.http://dx.doi.org/10.1080/10641963.2020.1833022renal sympathetic denervationspontaneous hypertensionleft ventricle hypertrophyraf/mek/erk |
| spellingShingle | Bing Xiao Fan Liu Ye-Hui Jin Ya-Qiong Jin Li Wang Jing-Chao Lu Xiu-Chun Yang Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf/MEK/ERK signaling pathway Clinical and Experimental Hypertension renal sympathetic denervation spontaneous hypertension left ventricle hypertrophy raf/mek/erk |
| title | Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf/MEK/ERK signaling pathway |
| title_full | Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf/MEK/ERK signaling pathway |
| title_fullStr | Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf/MEK/ERK signaling pathway |
| title_full_unstemmed | Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf/MEK/ERK signaling pathway |
| title_short | Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf/MEK/ERK signaling pathway |
| title_sort | renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the raf mek erk signaling pathway |
| topic | renal sympathetic denervation spontaneous hypertension left ventricle hypertrophy raf/mek/erk |
| url | http://dx.doi.org/10.1080/10641963.2020.1833022 |
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