Investigation of Serum Levels and Activity of Matrix Metalloproteinases 2 and 9 (MMP2, 9) in Opioid and Methamphetamine-Dependent Patients
Matrix metalloproteinases (MMPs) are a group of zinc-dependent proteolytic enzymes that play a role in extracellular matrix (mainly collagen) degradation and remodeling. MMPs are not only causes of the increase rewarding effects of drugs, but also act as pro-addictive agents. In this research, 22 mo...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2018-12-01
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| Series: | Acta Medica Iranica |
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| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/7396 |
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| author | Khadijeh Najafi Daniel Elieh Ali Komi Habibolah Khazaie Ali Moini Asad Vaisi-Raygani Hamid Reza Ahmadi Mohammad Rasoul Ghadami Amir Kiani |
| author_facet | Khadijeh Najafi Daniel Elieh Ali Komi Habibolah Khazaie Ali Moini Asad Vaisi-Raygani Hamid Reza Ahmadi Mohammad Rasoul Ghadami Amir Kiani |
| author_sort | Khadijeh Najafi |
| collection | DOAJ |
| description | Matrix metalloproteinases (MMPs) are a group of zinc-dependent proteolytic enzymes that play a role in extracellular matrix (mainly collagen) degradation and remodeling. MMPs are not only causes of the increase rewarding effects of drugs, but also act as pro-addictive agents. In this research, 22 morphine and 20 methamphetamine-dependent patients included and their serum levels and activity of MMP2 and 9 were assessed by ELISA and gelatin zymography and compared with those of 23 healthy individuals as a control group. Our findings showed a significant increase in serum levels and activity of MMP-2 in opium and methamphetamine groups in comparison with the control group. Moreover, unlike MMP-2, serum levels and activity of MMP-9 in both case groups found to be decreased. This study showed that long-term abuse of opium and methamphetamine changes the activity and serum levels of MMP9 and MMP2. The effects of methamphetamines and opium are associated with an increase in extracellular dopamine levels in the brain, achieved by facilitating the release of dopamine from pre-synaptic nerves. Our findings showed that serum levels and activities of MMP-9 and MMP-2 could be considered as alternative valuable biomarkers from those investigated of pro-addictive or anti-addictive factors in dependent patients. |
| first_indexed | 2024-12-12T00:25:14Z |
| format | Article |
| id | doaj.art-57eba7ce99024d1fa34f18b5d092504e |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-12T00:25:14Z |
| publishDate | 2018-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-57eba7ce99024d1fa34f18b5d092504e2022-12-22T00:44:39ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942018-12-015697396Investigation of Serum Levels and Activity of Matrix Metalloproteinases 2 and 9 (MMP2, 9) in Opioid and Methamphetamine-Dependent PatientsKhadijeh Najafi0Daniel Elieh Ali Komi1Habibolah Khazaie2Ali Moini3Asad Vaisi-Raygani4Hamid Reza Ahmadi5Mohammad Rasoul Ghadami6Amir Kiani7Pharmacy Student Research Committee, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. AND Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran.Sleep Disorders Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. AND Substance Abuse Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.Department of Internal Medicine, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.Department of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.Substance Abuse Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.Sleep Disorders Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.Department of Pharmacology and Toxicology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. AND Regenerative Medicine Research Center (RMRC), Kermanshah University of Medical Sciences, Kermanshah, Iran.Matrix metalloproteinases (MMPs) are a group of zinc-dependent proteolytic enzymes that play a role in extracellular matrix (mainly collagen) degradation and remodeling. MMPs are not only causes of the increase rewarding effects of drugs, but also act as pro-addictive agents. In this research, 22 morphine and 20 methamphetamine-dependent patients included and their serum levels and activity of MMP2 and 9 were assessed by ELISA and gelatin zymography and compared with those of 23 healthy individuals as a control group. Our findings showed a significant increase in serum levels and activity of MMP-2 in opium and methamphetamine groups in comparison with the control group. Moreover, unlike MMP-2, serum levels and activity of MMP-9 in both case groups found to be decreased. This study showed that long-term abuse of opium and methamphetamine changes the activity and serum levels of MMP9 and MMP2. The effects of methamphetamines and opium are associated with an increase in extracellular dopamine levels in the brain, achieved by facilitating the release of dopamine from pre-synaptic nerves. Our findings showed that serum levels and activities of MMP-9 and MMP-2 could be considered as alternative valuable biomarkers from those investigated of pro-addictive or anti-addictive factors in dependent patients.https://acta.tums.ac.ir/index.php/acta/article/view/7396Drug dependenceMatrix metalloproteinaseMorphineMethamphetamine |
| spellingShingle | Khadijeh Najafi Daniel Elieh Ali Komi Habibolah Khazaie Ali Moini Asad Vaisi-Raygani Hamid Reza Ahmadi Mohammad Rasoul Ghadami Amir Kiani Investigation of Serum Levels and Activity of Matrix Metalloproteinases 2 and 9 (MMP2, 9) in Opioid and Methamphetamine-Dependent Patients Acta Medica Iranica Drug dependence Matrix metalloproteinase Morphine Methamphetamine |
| title | Investigation of Serum Levels and Activity of Matrix Metalloproteinases 2 and 9 (MMP2, 9) in Opioid and Methamphetamine-Dependent Patients |
| title_full | Investigation of Serum Levels and Activity of Matrix Metalloproteinases 2 and 9 (MMP2, 9) in Opioid and Methamphetamine-Dependent Patients |
| title_fullStr | Investigation of Serum Levels and Activity of Matrix Metalloproteinases 2 and 9 (MMP2, 9) in Opioid and Methamphetamine-Dependent Patients |
| title_full_unstemmed | Investigation of Serum Levels and Activity of Matrix Metalloproteinases 2 and 9 (MMP2, 9) in Opioid and Methamphetamine-Dependent Patients |
| title_short | Investigation of Serum Levels and Activity of Matrix Metalloproteinases 2 and 9 (MMP2, 9) in Opioid and Methamphetamine-Dependent Patients |
| title_sort | investigation of serum levels and activity of matrix metalloproteinases 2 and 9 mmp2 9 in opioid and methamphetamine dependent patients |
| topic | Drug dependence Matrix metalloproteinase Morphine Methamphetamine |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/7396 |
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