AirID, a novel proximity biotinylation enzyme, for analysis of protein–protein interactions

AirID, a novel proximity biotinylation enzyme, for analysis of protein–protein interactions

Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism. However, there have been some improvements in the enzymes that are used for that purpose. Here, we...

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Bibliographic Details
Main Authors: Kohki Kido, Satoshi Yamanaka, Shogo Nakano, Kou Motani, Souta Shinohara, Akira Nozawa, Hidetaka Kosako, Sohei Ito, Tatsuya Sawasaki
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-05-01
Series:eLife
Subjects:
BioID
cell-free
protein-protein interaction
enzymatic engineering
ancestral sequence reconstruction
proximity labeling
Online Access:https://elifesciences.org/articles/54983

Internet

https://elifesciences.org/articles/54983

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