Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging.
The Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5-6 months and at 21-22 m...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5207734?pdf=render |
| _version_ | 1818856477294592000 |
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| author | Augusto Schneider Scot J Matkovich Berta Victoria Lina Spinel Andrzej Bartke Pawel Golusinski Michal M Masternak |
| author_facet | Augusto Schneider Scot J Matkovich Berta Victoria Lina Spinel Andrzej Bartke Pawel Golusinski Michal M Masternak |
| author_sort | Augusto Schneider |
| collection | DOAJ |
| description | The Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5-6 months and at 21-22 months of age for miRNA sequencing. We detected a total of 404 miRNAs in the ovarian samples, from which the abundance of 22 and 33 miRNAs changed with age in N and df/df mice, respectively. Of these, only three miRNAs were commonly regulated with age between N and df/df mice, indicating a very divergent miRNA profile between genotypes. We also detected that 46 miRNAs were regulated between N and df/df mice, of which 23 were regulated exclusively in young mice, 12 exclusively in old mice and 12 commonly regulated at young and old ages. Many genes likely to be targeted by these miRNAs are involved in the FoxO, mTOR, PI3k/Akt and insulin signaling pathways. These results suggest that the aging process has a differential impact on the ovarian miRNA profile in df/df mice, and suggest that these miRNAs can be central players in the maintenance of a younger ovarian phenotype. |
| first_indexed | 2024-12-19T08:25:07Z |
| format | Article |
| id | doaj.art-583363b154f54eef951f6984be5783ee |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T08:25:07Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-583363b154f54eef951f6984be5783ee2022-12-21T20:29:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e016921310.1371/journal.pone.0169213Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging.Augusto SchneiderScot J MatkovichBerta VictoriaLina SpinelAndrzej BartkePawel GolusinskiMichal M MasternakThe Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5-6 months and at 21-22 months of age for miRNA sequencing. We detected a total of 404 miRNAs in the ovarian samples, from which the abundance of 22 and 33 miRNAs changed with age in N and df/df mice, respectively. Of these, only three miRNAs were commonly regulated with age between N and df/df mice, indicating a very divergent miRNA profile between genotypes. We also detected that 46 miRNAs were regulated between N and df/df mice, of which 23 were regulated exclusively in young mice, 12 exclusively in old mice and 12 commonly regulated at young and old ages. Many genes likely to be targeted by these miRNAs are involved in the FoxO, mTOR, PI3k/Akt and insulin signaling pathways. These results suggest that the aging process has a differential impact on the ovarian miRNA profile in df/df mice, and suggest that these miRNAs can be central players in the maintenance of a younger ovarian phenotype.http://europepmc.org/articles/PMC5207734?pdf=render |
| spellingShingle | Augusto Schneider Scot J Matkovich Berta Victoria Lina Spinel Andrzej Bartke Pawel Golusinski Michal M Masternak Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging. PLoS ONE |
| title | Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging. |
| title_full | Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging. |
| title_fullStr | Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging. |
| title_full_unstemmed | Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging. |
| title_short | Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging. |
| title_sort | changes of ovarian microrna profile in long living ames dwarf mice during aging |
| url | http://europepmc.org/articles/PMC5207734?pdf=render |
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