A feeder- and xeno-free human induced pluripotent stem cell line obtained from primary human dermal fibroblasts with epigenetic repression of reprogramming factors expression: GPCCi001-A

A feeder- and xeno-free human induced pluripotent stem cell line obtained from primary human dermal fibroblasts with epigenetic repression of reprogramming factors expression: GPCCi001-A

The primary human dermal fibroblasts (PHDFs) from breast cancer patient were obtained to generate the human induced pluripotent stem cell line GPCCi001-A via lentiviral transfection. Thus, a modified EF1a-hSTEMCCA-loxP with tetO operator which regulates transgene expression was used. This method tak...

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Bibliographic Details
Main Authors: Michał Stefan Lach, Joanna P. Wroblewska, Ewelina Augustyniak, Katarzyna Kulcenty, Wiktoria Maria Suchorska
Format: Article
Language:English
Published: Elsevier 2017-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506117300181

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http://www.sciencedirect.com/science/article/pii/S1873506117300181

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